LOCUS       Exported                3321 bp ds-DNA     circular SYN 17-JUL-2006
DEFINITION  Complementary copy of pDONR221-P1P4 rc.
ACCESSION   .
VERSION     .
KEYWORDS    pDONR221-P1P4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Invitrogen
  TITLE     Direct Submission
  JOURNAL   Exported Jan 17, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=Untitled Entry Clone CMV promoter 
                     (JH103) L1-L4"
     misc_feature    complement(268..295)
                     /label=rrnB T2 transcription termination sequence
                     /note="rrnB T2 transcription termination sequence"
     misc_feature    complement(427..470)
                     /label=rrnB T1 transcription termination sequence
                     /note="rrnB T1 transcription termination sequence"
     primer_bind     517..533
                     /label=M13 Forward (-40) primer binding site
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     537..552
                     /label=M13 Forward (-20) primer binding site
                     /note="M13 Forward (-20) primer binding site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1 recombination site"
                     /note="recognized as attL1"
     promoter        670..1428
                     /note="CMV promoter"
     misc_recomb     complement(1433..1531)
                     /label=attL4
                     /note="attL4 recombination site"
                     /note="recognized as attL4"
     misc_feature    join(1443..3321,1..658)
                     /label=nonstandard type: vector
                     /note="/source=pDONR221-P1P4"
                     /note="/type=Donor Vector"
     primer_bind     complement(1586..1602)
                     /label=M13 Reverse Primer Binding Site
                     /note="M13 Reverse Primer Binding Site"
     gene            1715..2524
                     /label=Kanamycin resistance gene
                     /note="Kanamycin resistance gene"
     rep_origin      2645..3318
                     /label=f1 origin
                     /note="f1 origin"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctta cgatctatac attgaatcaa tattggcaat tagccatatt agtcattggt
      721 tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat
      781 atgtacattt atattggctc atgtccaata tgaccgccat gttgacattg attattgact
      841 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      901 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      961 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     1021 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     1081 agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     1141 atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     1201 atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga
     1261 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     1321 gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta
     1381 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatccg gcacccaact
     1441 tttctataca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
     1501 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgcagggcg gccgcgatat
     1561 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     1621 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
     1681 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
     1741 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     1801 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
     1861 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     1921 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     1981 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga
     2041 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     2101 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     2161 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     2221 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
     2281 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     2341 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     2401 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     2461 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     2521 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga
     2581 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     2641 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2701 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2761 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2821 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2881 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2941 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3001 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3061 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3121 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3181 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3241 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3301 ctggcctttt gctcacatgt t
//
