LOCUS       Exported                2992 bp ds-DNA     circular SYN 17-JUL-2006
DEFINITION  Complementary copy of pDONR221-P1P4 rc.
ACCESSION   .
VERSION     .
KEYWORDS    pDONR221-P1P4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Invitrogen
  TITLE     Direct Submission
  JOURNAL   Exported Jan 17, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=Untitled Entry Clone Untitled L1-L4"
     misc_feature    complement(268..295)
                     /label=rrnB T2 transcription termination sequence
                     /note="rrnB T2 transcription termination sequence"
     misc_feature    complement(427..470)
                     /label=rrnB T1 transcription termination sequence
                     /note="rrnB T1 transcription termination sequence"
     primer_bind     517..533
                     /label=M13 Forward (-40) primer binding site
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     537..552
                     /label=M13 Forward (-20) primer binding site
                     /note="M13 Forward (-20) primer binding site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1 recombination site"
                     /note="recognized as attL1"
     promoter        674..1103
                     /note="RIP"
                     /note="Rat Insulin I Promoter, pancreatic islet beta-cell 
                     specific"
     misc_feature    1085..1102
                     /label=+1-18 of rat Ins1 primary transcript
                     /note="+1-18 of rat Ins1 primary transcript"
     misc_recomb     complement(1104..1202)
                     /label=attL4
                     /note="attL4 recombination site"
                     /note="recognized as attL4"
     misc_feature    join(1114..2992,1..658)
                     /label=nonstandard type: vector
                     /note="/source=pDONR221-P1P4"
                     /note="/type=Donor Vector"
     primer_bind     complement(1257..1273)
                     /label=M13 Reverse Primer Binding Site
                     /note="M13 Reverse Primer Binding Site"
     gene            1386..2195
                     /label=Kanamycin resistance gene
                     /note="Kanamycin resistance gene"
     rep_origin      2316..2989
                     /label=f1 origin
                     /note="f1 origin"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctta agactgagct aagaatccag ctatcaatag aaactatgaa acagttccag
      721 ggacaaagat accaggtccc caacaactgc aactttctgg gaaatgaggt ggaaaatgct
      781 cagccaagga aaaagagggc cttaccctct ctgggacaat gattgtgctg tgaactgctt
      841 catcaggcca tctggcccct tgttaataat ctaattaccc taggtctaag tagagttgtt
      901 gacgtccaat gagcgctttc tgcagactta gcactaggca agtgtttgga aattacagct
      961 tcagcccctc tcgccatctg cctacctacc cctcctagag cccttaatgg gccaaacggc
     1021 aaagtccagg gggcagagag gaggtgcttt ggactataaa gctagtggag acccagtaac
     1081 tcccaaccct aagtgaccag ctcacccaac ttttctatac aaagttggca ttataagaaa
     1141 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     1201 ttgccatcca gctgcagggc ggccgcgata tcccctatag tgagtcgtat tacatggtca
     1261 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
     1321 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
     1381 gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg
     1441 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
     1501 atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
     1561 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
     1621 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
     1681 tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
     1741 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
     1801 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
     1861 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
     1921 aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
     1981 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     2041 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     2101 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     2161 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
     2221 actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta
     2281 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2341 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2401 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2461 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     2521 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2581 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2641 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2701 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2761 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2821 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2881 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2941 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
