LOCUS pDONR221-P1P5r 4765 bp DNA circular SYN 23-APR-2009 DEFINITION Complementary copy of pDONR221-P1P5r. SOURCE ORGANISM COMMENT VNTDATE|399123546| COMMENT VNTDBDATE|416243884| COMMENT VNTNAME|pDONR221-P1P5r| COMMENT VNTAUTHORNAME|Invitrogen| COMMENT VNTAUTHORTEL|(800)-357-3114| COMMENT VNTAUTHORFAX|(760)-602-6500| COMMENT VNTOAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com| COMMENT VNTAUTHOREML|bioinfosales@invitrogen.com| COMMENT VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics| COMMENT VNTAUTHORAD1|Invitrogen Corporation| COMMENT VNTAUTHORAD2|1620 Faraday Avenue| COMMENT VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.| FEATURES Location/Qualifiers misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence" misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence" primer_bind 537..552 /note="M13 Forward (-20) primer binding site" primer_bind 517..533 /note="M13 Forward (-40) primer binding site" primer_bind complement(1334..1350) /note="M13 Reverse primer binding site" rep_origin 2393..3066 /note="f1 origin" gene 1463..2272 /note="Kanamycin resistance gene" misc_recomb 570..668 /label=attL1 /note="attL1 recombination site" /note="recognized as attL1" misc_recomb 1132..1288 /label=attL5r /note="attL5r recombination site" /note="recognized as attR5" promoter 669..1128 /note="TBG promoter" /note="Thyroxine binding globin promoter, liver specific promoter" vector join(1139..3069,1..658) /source="pDONR221-P1P5r" /type="Donor Vector" source 1..3069 /dnas_title="Untitled Entry Clone Untitled Entry Clone Addgene_61593_AAV_TBG_SaCas9_OLLAS_U6_sgRNA L1-L4 L1-L5" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaAA 661 AGCAGGCTgg gctggaagct acctttgaca tcatttcctc tgcgaatgca tgtataattt 721 ctacagaacc tattagaaag gatcacccag cctctgcttt tgtacaactt tcccttaaaa 781 aactgccaat tccactgctg tttggcccaa tagtgagaac tttttcctgc tgcctcttgg 841 tgcttttgcc tatggcccct attctgcctg ctgaagacac tcttgccagc atggacttaa 901 acccctccag ctctgacaat cctctttctc ttttgtttta catgaagggt ctggcagcca 961 aagcaatcac tcaaagttca aaccttatca ttttttgctt tgttcctctt ggccttggtt 1021 ttgtacatca gctttgaaaa taccatccca gggttaatgc tggggttaat ttataactaa 1081 gagtgctcta gttttgcaat acaggacatg ctataaaaat ggaaagatac cCAACTTTgt 1141 atacaaaagt tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt 1201 tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca ctatgaatca 1261 actacttaga tggtattagt gacctgtact gcagggcggc cgcgatatcc cctatagtga 1321 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg 1381 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat 1441 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt 1501 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca 1561 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa 1621 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct 1681 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg 1741 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga 1801 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc 1861 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg 1921 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt 1981 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac 2041 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat 2101 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg 2161 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa 2221 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt 2281 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc 2341 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga 2401 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 2461 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 2521 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 2581 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 2641 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 2701 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 2761 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 2821 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 2881 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 2941 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 3001 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 3061 tcacatgtt //