LOCUS       pDONR221-P1P5r          4765 bp    DNA     circular SYN 23-APR-2009
DEFINITION  Complementary copy of pDONR221-P1P5r.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|399123546|
COMMENT     VNTDBDATE|416243884|
COMMENT     VNTNAME|pDONR221-P1P5r|
COMMENT     VNTAUTHORNAME|Invitrogen|
COMMENT     VNTAUTHORTEL|(800)-357-3114|
COMMENT     VNTAUTHORFAX|(760)-602-6500|
COMMENT     VNTOAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com|
COMMENT     VNTAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics|
COMMENT     VNTAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
FEATURES             Location/Qualifiers
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence"
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence"
     primer_bind     537..552
                     /note="M13 Forward (-20) primer binding site"
     primer_bind     517..533
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     complement(1286..1302)
                     /note="M13 Reverse primer binding site"
     rep_origin      2345..3018
                     /note="f1 origin"
     gene            1415..2224
                     /note="Kanamycin resistance gene"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1 recombination site"
                     /note="recognized as attL1"
     misc_recomb     1084..1240
                     /label=attL5r
                     /note="attL5r recombination site"
                     /note="recognized as attR5"
     promoter        669..1075
                     /note="cTnT promoter"
                     /note="Chicken cardiac trophonin T promoter, cardiac
                     specific"
     vector          join(1091..3021,1..658)
                     /source="pDONR221-P1P5r"
                     /type="Donor Vector"
     source          1..3021
                     /dnas_title="Untitled Entry Clone Untitled Entry Clone
                     pAcTnTS_heart_promoter L1-L4 L1-L5"
ORIGIN      
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaAA
      661 AGCAGGCTGC AGTCTGGGCT TTCACAAGAC AGCATCTGGG GCTGCGGCAG AGGGTCGGGT
      721 CCGAAGCGCT GCCTTATCAG CGTCCCCAGC CCTGGGAGGT GACAGCTGGC TGGCTTGTGT
      781 CAGCCCCTCG GGCACTCACG TATCTCCGTC CGACGGGTTT AAAATAGCAA AACTCTGAGG
      841 CCACACAATA GCTTGGGCTT ATATGGGCTC CTGTGGGGGA AGGGGGAGCA CGGAGGGGGC
      901 CGGGGCCGCT GCTGCCAAAA TAGCAGCTCA CAAGTGTTGC ATTCCTCTCT GGGCGCCGGG
      961 CACATTCCTG CTGGCTCTGC CCGCCCCGGG GTGGGCGCCG GGGGGACCTT AAAGCCTCTG
     1021 CCCCCCAAGG AGCCCTTCCC AGACAGCCGC CGGCACCCAC CGCTCCGTGG GACCTAAGCT
     1081 TGCCAACTTT gtatacaaaa gttgaacgag aaacgtaaaa tgatataaat atcaatatat
     1141 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt
     1201 cactatgaat caactactta gatggtatta gtgacctgta ctgcagggcg gccgcgatat
     1261 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     1321 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
     1381 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
     1441 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     1501 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
     1561 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     1621 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     1681 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga
     1741 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     1801 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     1861 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     1921 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
     1981 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     2041 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     2101 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     2161 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     2221 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga
     2281 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     2341 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2401 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2461 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2521 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2581 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2641 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2701 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2761 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2821 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2881 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2941 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3001 ctggcctttt gctcacatgt t
//

