LOCUS Exported 2665 bp ds-DNA circular SYN 17-JUL-2006 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pDONR221-P5P4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2665) AUTHORS Invitrogen TITLE Direct Submission JOURNAL Exported Jan 17, 2019 from SnapGene Viewer 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2665 /organism="synthetic DNA construct" /note="/dnas_title=Untitled Entry Clone 5_4_HA L5-L4" misc_feature complement(268..295) /label=rrnB T2 transcription termination sequence /note="rrnB T2 transcription termination sequence" misc_feature complement(427..470) /label=rrnB T1 transcription termination sequence /note="rrnB T1 transcription termination sequence" primer_bind 517..533 /label=M13 Forward (-40) primer binding site /note="M13 Forward (-40) primer binding site" primer_bind 537..552 /label=M13 Forward (-20) primer binding site /note="M13 Forward (-20) primer binding site" misc_recomb 570..665 /label=attL5 /note="attL5 recombination site" /note="recognized as attL5" 5'UTR 668..733 /label=Xenopus 5' beta-globin leader /note="Xenopus 5' beta-globin leader" /note="M17374.2" /note="enhances translation" gene 734..763 /label=HA epitope tag /note="HA epitope tag" misc_feature 764..775 /label=GGGS linker /note="GGGS linker" misc_recomb complement(777..875) /label=attL4 /note="attL4 recombination site" /note="recognized as attL4" misc_feature join(787..2665,1..658) /label=nonstandard type: vector /note="/source=pDONR221-P5P4" /note="/type=Donor Vector" primer_bind complement(930..946) /label=M13 Reverse primer binding site /note="M13 Reverse primer binding site" gene 1059..1868 /label=Kanamycin resistance gene /note="Kanamycin resistance gene" rep_origin 1989..2662 /label=f1 origin /note="f1 origin" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtatacaaa 661 agttgtagaa tacaagcttc ttgttctttt tgcagaagct cagaataaac gctcaacttt 721 ggcagatctg aacatgtacc catacgatgt gccagattac gctggaggcg gatcccaccc 781 aacttttcta tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga 841 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgcag ggcggccgcg 901 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg 961 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa 1021 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa 1081 cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc 1141 gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc 1201 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg 1261 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta 1321 ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat 1381 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc 1441 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg 1501 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc 1561 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac 1621 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga 1681 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg 1741 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa 1801 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt 1861 ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac 1921 gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag 1981 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 2041 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 2101 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 2161 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 2221 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 2281 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 2341 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 2401 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 2461 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 2521 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 2581 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 2641 tttgctggcc ttttgctcac atgtt //