LOCUS       pDONR221-P5P4           4774 bp    DNA     circular SYN 23-APR-2009
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|391339046|
COMMENT     VNTDBDATE|416244910|
COMMENT     VNTNAME|pDONR221-P5P4|
COMMENT     VNTAUTHORNAME|Invitrogen|
COMMENT     VNTAUTHORTEL|(800)-357-3114|
COMMENT     VNTAUTHORFAX|(760)-602-6500|
COMMENT     VNTOAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com|
COMMENT     VNTAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics|
COMMENT     VNTAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
FEATURES             Location/Qualifiers
     misc_recomb     570..665
                     /label=attL5
                     /note="attL5 recombination site"
                     /note="recognized as attL5"
     misc_recomb     complement(1518..1616)
                     /label=attL4
                     /note="attL4 recombination site"
                     /note="recognized as attL4"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence"
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence"
     primer_bind     537..552
                     /note="M13 Forward (-20) primer binding site"
     primer_bind     517..533
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     complement(1671..1687)
                     /note="M13 Reverse primer binding site"
     rep_origin      2730..3403
                     /note="f1 origin"
     gene            1800..2609
                     /note="Kanamycin resistance gene"
     5'UTR           668..733
                     /note="Xenopus 5' beta-globin leader"
                     /note="M17374.2"
                     /note="enhances translation"
     misc_feature    1445..1453
                     /note="GSG linker"
     misc_feature    1454..1510
                     /note="P2A self-cleaving peptide"
     misc_feature    1505..1510
                     /note="G^P cleavage site"
     vector          join(1528..3406,1..658)
                     /source="pDONR221-P5P4"
                     /type="Donor Vector"
     gene            734..1441
                     /note="mCherry"
     source          1..3406
                     /dnas_title="KP301_mCherry_P2A"
ORIGIN      
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtatacaAA
      661 AGTTGTAgaa tacaagcttc ttgttctttt tgcagaagct cagaataaac gctcaacttt
      721 ggcagatctg aacatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
      781 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
      841 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      901 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
      961 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     1021 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     1081 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     1141 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     1201 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     1261 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     1321 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     1381 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     1441 gGCCGGATCC GGAGCTACTA ATTTCTCTCT GTTAAAGCAG GCAGGAGATG TGGAGGAGAA
     1501 TCCAGGTCCT aaggccCACC CAACTTTtct atacaaagtt ggcattataa gaaagcattg
     1561 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     1621 tccagctgca gggcggccgc gatatcccct atagtgagtc gtattacatg gtcatagctg
     1681 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     1741 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
     1801 tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt
     1861 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct
     1921 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
     1981 atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga
     2041 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg
     2101 gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
     2161 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca
     2221 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg
     2281 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
     2341 ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
     2401 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
     2461 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
     2521 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     2581 ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg taacactggc
     2641 agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga
     2701 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2761 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2821 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2881 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     2941 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3001 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3061 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3121 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3181 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3241 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3301 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3361 cctttttacg gttcctggcc ttttgctggc cttttgctca catgtt
//

