LOCUS       pDONR221-P5P4           4774 bp    DNA     circular SYN 23-APR-2009
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|391339046|
COMMENT     VNTDBDATE|416244910|
COMMENT     VNTNAME|pDONR221-P5P4|
COMMENT     VNTAUTHORNAME|Invitrogen|
COMMENT     VNTAUTHORTEL|(800)-357-3114|
COMMENT     VNTAUTHORFAX|(760)-602-6500|
COMMENT     VNTOAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com|
COMMENT     VNTAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics|
COMMENT     VNTAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
FEATURES             Location/Qualifiers
     misc_recomb     570..665
                     /label=attL5
                     /note="attL5 recombination site"
                     /note="recognized as attL5"
     misc_recomb     complement(1527..1625)
                     /label=attL4
                     /note="attL4 recombination site"
                     /note="recognized as attL4"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence"
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence"
     primer_bind     537..552
                     /note="M13 Forward (-20) primer binding site"
     primer_bind     517..533
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     complement(1680..1696)
                     /note="M13 Reverse primer binding site"
     rep_origin      2739..3412
                     /note="f1 origin"
     gene            1809..2618
                     /note="Kanamycin resistance gene"
     5'UTR           668..733
                     /note="Xenopus 5' beta-globin leader"
                     /note="M17374.2"
                     /note="enhances translation"
     misc_feature    1454..1462
                     /note="GSG linker"
     misc_feature    1463..1519
                     /note="P2A self-cleaving peptide"
     misc_feature    1514..1519
                     /note="G^P cleavage site"
     vector          join(1537..3415,1..658)
                     /source="pDONR221-P5P4"
                     /type="Donor Vector"
     gene            734..1450
                     /note="eCFP"
     source          1..3415
                     /dnas_title="KP401_CFP_P2A"
ORIGIN      
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtatacaAA
      661 AGTTGTAgaa tacaagcttc ttgttctttt tgcagaagct cagaataaac gctcaacttt
      721 ggcagatctg aacatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      781 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      841 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      901 gccctggccc accctcgtga ccaccctgac ctggggcgtg cagtgcttca gccgctaccc
      961 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     1021 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     1081 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     1141 catcctgggg cacaagctgg agtacaacta catcagccac aacgtctata tcaccgccga
     1201 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag
     1261 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     1321 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     1381 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     1441 gctgtacaag GCCGGATCCG GAGCTACTAA TTTCTCTCTG TTAAAGCAGG CAGGAGATGT
     1501 GGAGGAGAAT CCAGGTCCTa aggccCACCC AACTTTtcta tacaaagttg gcattataag
     1561 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     1621 tatttgccat ccagctgcag ggcggccgcg atatccccta tagtgagtcg tattacatgg
     1681 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     1741 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     1801 ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
     1861 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     1921 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     1981 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     2041 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     2101 cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     2161 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     2221 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     2281 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     2341 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     2401 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     2461 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     2521 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     2581 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
     2641 aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc
     2701 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2761 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2821 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2881 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2941 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3001 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3061 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3121 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3181 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3241 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3301 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3361 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//

