LOCUS Exported 3094 bp ds-DNA circular SYN 17-JUL-2006 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pDONR221-P5P4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3094) AUTHORS Invitrogen TITLE Direct Submission JOURNAL Exported Jan 17, 2019 from SnapGene Viewer 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3094 /organism="synthetic DNA construct" /note="/dnas_title=KO901_Blast_P2A" misc_feature complement(268..295) /label=rrnB T2 transcription termination sequence /note="rrnB T2 transcription termination sequence" misc_feature complement(427..470) /label=rrnB T1 transcription termination sequence /note="rrnB T1 transcription termination sequence" primer_bind 517..533 /label=M13 Forward (-40) primer binding site /note="M13 Forward (-40) primer binding site" primer_bind 537..552 /label=M13 Forward (-20) primer binding site /note="M13 Forward (-20) primer binding site" misc_recomb 570..665 /label=attL5 /note="attL5 recombination site" /note="recognized as attL5" 5'UTR 668..733 /label=Xenopus 5' beta-globin leader /note="Xenopus 5' beta-globin leader" /note="M17374.2" /note="enhances translation" gene 734..1129 /label=Blasticidin resistance gene /note="Blasticidin resistance gene" misc_feature 1133..1141 /label=GSG linker /note="GSG linker" misc_feature 1142..1198 /label=P2A self-cleaving peptide /note="P2A self-cleaving peptide" misc_feature 1193..1198 /label=G^P cleavage site /note="G^P cleavage site" misc_recomb complement(1206..1304) /label=attL4 /note="attL4 recombination site" /note="recognized as attL4" misc_feature join(1216..3094,1..658) /label=nonstandard type: vector /note="/source=pDONR221-P5P4" /note="/type=Donor Vector" primer_bind complement(1359..1375) /label=M13 Reverse primer binding site /note="M13 Reverse primer binding site" gene 1488..2297 /label=Kanamycin resistance gene /note="Kanamycin resistance gene" rep_origin 2418..3091 /label=f1 origin /note="f1 origin" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtatacaaa 661 agttgtagaa tacaagcttc ttgttctttt tgcagaagct cagaataaac gctcaacttt 721 ggcagatctg aacatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc 781 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct 841 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg 901 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat 961 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt 1021 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac 1081 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggcg ccggatccgg 1141 agctactaat ttctctctgt taaagcaggc aggagatgtg gaggagaatc caggtcctaa 1201 ggcccaccca acttttctat acaaagttgg cattataaga aagcattgct tatcaatttg 1261 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgcagg 1321 gcggccgcga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc 1381 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat 1441 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc 1501 aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata 1561 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc 1621 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag 1681 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt 1741 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat 1801 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt 1861 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat 1921 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg 1981 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc 2041 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg 2101 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc 2161 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc 2221 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc 2281 tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag agcattacgc 2341 tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt 2401 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 2461 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 2521 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 2581 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 2641 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 2701 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 2761 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 2821 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 2881 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 2941 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 3001 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 3061 tcctggcctt ttgctggcct tttgctcaca tgtt //