LOCUS       Exported                3094 bp ds-DNA     circular SYN 17-JUL-2006
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDONR221-P5P4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Invitrogen
  TITLE     Direct Submission
  JOURNAL   Exported Jan 17, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=KO901_Blast_P2A"
     misc_feature    complement(268..295)
                     /label=rrnB T2 transcription termination sequence
                     /note="rrnB T2 transcription termination sequence"
     misc_feature    complement(427..470)
                     /label=rrnB T1 transcription termination sequence
                     /note="rrnB T1 transcription termination sequence"
     primer_bind     517..533
                     /label=M13 Forward (-40) primer binding site
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     537..552
                     /label=M13 Forward (-20) primer binding site
                     /note="M13 Forward (-20) primer binding site"
     misc_recomb     570..665
                     /label=attL5
                     /note="attL5 recombination site"
                     /note="recognized as attL5"
     5'UTR           668..733
                     /label=Xenopus 5' beta-globin leader
                     /note="Xenopus 5' beta-globin leader"
                     /note="M17374.2"
                     /note="enhances translation"
     gene            734..1129
                     /label=Blasticidin resistance gene
                     /note="Blasticidin resistance gene"
     misc_feature    1133..1141
                     /label=GSG linker
                     /note="GSG linker"
     misc_feature    1142..1198
                     /label=P2A self-cleaving peptide
                     /note="P2A self-cleaving peptide"
     misc_feature    1193..1198
                     /label=G^P cleavage site
                     /note="G^P cleavage site"
     misc_recomb     complement(1206..1304)
                     /label=attL4
                     /note="attL4 recombination site"
                     /note="recognized as attL4"
     misc_feature    join(1216..3094,1..658)
                     /label=nonstandard type: vector
                     /note="/source=pDONR221-P5P4"
                     /note="/type=Donor Vector"
     primer_bind     complement(1359..1375)
                     /label=M13 Reverse primer binding site
                     /note="M13 Reverse primer binding site"
     gene            1488..2297
                     /label=Kanamycin resistance gene
                     /note="Kanamycin resistance gene"
     rep_origin      2418..3091
                     /label=f1 origin
                     /note="f1 origin"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtatacaaa
      661 agttgtagaa tacaagcttc ttgttctttt tgcagaagct cagaataaac gctcaacttt
      721 ggcagatctg aacatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc
      781 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct
      841 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
      901 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
      961 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt
     1021 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac
     1081 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggcg ccggatccgg
     1141 agctactaat ttctctctgt taaagcaggc aggagatgtg gaggagaatc caggtcctaa
     1201 ggcccaccca acttttctat acaaagttgg cattataaga aagcattgct tatcaatttg
     1261 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgcagg
     1321 gcggccgcga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     1381 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     1441 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
     1501 aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
     1561 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc
     1621 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
     1681 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
     1741 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat
     1801 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
     1861 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
     1921 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
     1981 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc
     2041 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
     2101 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     2161 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     2221 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
     2281 tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag agcattacgc
     2341 tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt
     2401 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2461 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2521 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2581 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2641 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2701 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2761 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2821 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2881 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2941 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3001 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3061 tcctggcctt ttgctggcct tttgctcaca tgtt
//
