LOCUS       pDONR221-P5P4           4774 bp    DNA     circular SYN 23-APR-2009
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|391339046|
COMMENT     VNTDBDATE|416244910|
COMMENT     VNTNAME|pDONR221-P5P4|
COMMENT     VNTAUTHORNAME|Invitrogen|
COMMENT     VNTAUTHORTEL|(800)-357-3114|
COMMENT     VNTAUTHORFAX|(760)-602-6500|
COMMENT     VNTOAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com|
COMMENT     VNTAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics|
COMMENT     VNTAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
FEATURES             Location/Qualifiers
     misc_recomb     570..665
                     /label=attL5
                     /note="attL5 recombination site"
                     /note="recognized as attL5"
     misc_recomb     complement(1407..1505)
                     /label=attL4
                     /note="attL4 recombination site"
                     /note="recognized as attL4"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence"
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence"
     primer_bind     537..552
                     /note="M13 Forward (-20) primer binding site"
     primer_bind     517..533
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     complement(1560..1576)
                     /note="M13 Reverse primer binding site"
     rep_origin      2619..3292
                     /note="f1 origin"
     gene            1689..2498
                     /note="Kanamycin resistance gene"
     5'UTR           668..733
                     /note="Xenopus 5' beta-globin leader"
                     /note="M17374.2"
                     /note="enhances translation"
     misc_feature    1334..1342
                     /note="GSG linker"
     misc_feature    1343..1399
                     /note="P2A self-cleaving peptide"
     misc_feature    1394..1399
                     /note="G^P cleavage site"
     vector          join(1417..3295,1..658)
                     /source="pDONR221-P5P4"
                     /type="Donor Vector"
     gene            734..1330
                     /note="Puromycin resistance gene"
     source          1..3295
                     /dnas_title="KP101_Puro_P2A"
ORIGIN      
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtatacaAA
      661 AGTTGTAgaa tacaagcttc ttgttctttt tgcagaagct cagaataaac gctcaacttt
      721 ggcagatctg aacatgaccg agtacaagcc taccgtgcgc ctggccactc gcgatgatgt
      781 gccccgcgcc gtccgcactc tggccgccgc tttcgccgac taccccgcta cccggcacac
      841 cgtggacccc gaccggcaca tcgagcgtgt gacagagttg caggagctgt tcctgacccg
      901 cgtcgggctg gacatcggca aggtgtgggt agccgacgac ggcgcggccg tggccgtgtg
      961 gactaccccc gagagcgttg aggccggcgc cgtgttcgcc gagatcggcc cccgaatggc
     1021 cgagctgagc ggcagccgcc tggccgccca gcagcaaatg gagggcctgc ttgcccccca
     1081 tcgtcccaag gagcctgcct ggtttctggc cactgtagga gtgagccccg accaccaggg
     1141 caagggcttg ggcagcgccg tcgtgttgcc cggcgtagag gccgccgaac gcgccggtgt
     1201 gcccgccttt ctcgaaacaa gcgcaccaag aaaccttcca ttctacgagc gcctgggctt
     1261 caccgtgacc gccgatgtcg aggtgcccga gggacctagg acctggtgta tgacacgaaa
     1321 acctggcgcc GCCGGATCCG GAGCTACTAA TTTCTCTCTG TTAAAGCAGG CAGGAGATGT
     1381 GGAGGAGAAT CCAGGTCCTa aggccCACCC AACTTTtcta tacaaagttg gcattataag
     1441 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     1501 tatttgccat ccagctgcag ggcggccgcg atatccccta tagtgagtcg tattacatgg
     1561 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     1621 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     1681 ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
     1741 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     1801 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     1861 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     1921 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     1981 cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     2041 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     2101 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     2161 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     2221 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     2281 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     2341 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     2401 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     2461 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
     2521 aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc
     2581 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2641 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2701 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2761 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2821 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2881 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2941 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3001 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3061 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3121 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3181 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3241 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//

