LOCUS pDONR221-P5P4 4774 bp DNA circular SYN 23-APR-2009 SOURCE ORGANISM COMMENT VNTDATE|391339046| COMMENT VNTDBDATE|416244910| COMMENT VNTNAME|pDONR221-P5P4| COMMENT VNTAUTHORNAME|Invitrogen| COMMENT VNTAUTHORTEL|(800)-357-3114| COMMENT VNTAUTHORFAX|(760)-602-6500| COMMENT VNTOAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com| COMMENT VNTAUTHOREML|bioinfosales@invitrogen.com| COMMENT VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics| COMMENT VNTAUTHORAD1|Invitrogen Corporation| COMMENT VNTAUTHORAD2|1620 Faraday Avenue| COMMENT VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.| FEATURES Location/Qualifiers misc_recomb 570..665 /label=attL5 /note="attL5 recombination site" /note="recognized as attL5" misc_recomb complement(969..1067) /label=attL4 /note="attL4 recombination site" /note="recognized as attL4" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence" misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence" primer_bind 537..552 /note="M13 Forward (-20) primer binding site" primer_bind 517..533 /note="M13 Forward (-40) primer binding site" primer_bind complement(1122..1138) /note="M13 Reverse primer binding site" rep_origin 2181..2854 /note="f1 origin" gene 1251..2060 /note="Kanamycin resistance gene" 5'UTR 668..733 /note="Xenopus 5' beta-globin leader" /note="M17374.2" /note="enhances translation" vector join(979..2857,1..658) /source="pDONR221-P5P4" /type="Donor Vector" misc_feature 944..961 /note="GSGSGS linker" misc_feature 740..943 /note="mAID " /note="Described in PMID: 27052166" source 1..2857 /dnas_title="KW701_mAID" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtatacaAA 661 AGTTGTAgaa tacaagcttc ttgttctttt tgcagaagct cagaataaac gctcaacttt 721 ggcagatctg aacatgggcA AGGAGAAGAG TGCTTGTCCT AAAGATCCAG CCAAACCTCC 781 GGCCAAGGCA CAAGTTGTGG GATGGCCACC GGTGAGATCA TACCGGAAGA ACGTGATGGT 841 TTCCTGCCAA AAATCAAGCG GTGGCCCGGA GGCGGCGGCG TTCGTGAAGG TATCAATGGA 901 CGGAGCACCG TACTTGAGGA AAATCGATTT GAGGATGTAT AAAggcagtG GatccggAtc 961 TaaggccCAC CCAACTTTtc tatacaaagt tggcattata agaaagcatt gcttatcaat 1021 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctgc 1081 agggcggccg cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc 1141 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat 1201 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata 1261 ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt 1321 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga 1381 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta 1441 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc 1501 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag 1561 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag 1621 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg 1681 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt 1741 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt 1801 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt 1861 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat 1921 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac 1981 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga 2041 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta 2101 cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt 2161 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 2221 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 2281 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 2341 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 2401 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 2461 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 2521 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 2581 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 2641 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 2701 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 2761 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 2821 ggttcctggc cttttgctgg ccttttgctc acatgtt //