LOCUS Exported 3319 bp ds-DNA circular SYN 17-JUL-2006 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pDONR221-P5P4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3319) AUTHORS Invitrogen TITLE Direct Submission JOURNAL Exported Jan 17, 2019 from SnapGene Viewer 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3319 /organism="synthetic DNA construct" /note="/dnas_title=Untitled Entry Clone Untitled Entry Clone Addgene_49386_APEX2_NES L5-L2 L5-L4" misc_feature complement(268..295) /label=rrnB T2 transcription termination sequence /note="rrnB T2 transcription termination sequence" misc_feature complement(427..470) /label=rrnB T1 transcription termination sequence /note="rrnB T1 transcription termination sequence" primer_bind 517..533 /label=M13 Forward (-40) primer binding site /note="M13 Forward (-40) primer binding site" primer_bind 537..552 /label=M13 Forward (-20) primer binding site /note="M13 Forward (-20) primer binding site" misc_recomb 570..665 /label=attL5 /note="attL5 recombination site" /note="recognized as attL5" gene 674..1414 /label=APEX2 /note="APEX2" misc_recomb complement(1431..1529) /label=attL4 /note="attL4 recombination site" /note="recognized as attL4" misc_feature join(1441..3319,1..658) /label=nonstandard type: vector /note="/source=pDONR221-P5P4" /note="/type=Donor Vector" primer_bind complement(1584..1600) /label=M13 Reverse primer binding site /note="M13 Reverse primer binding site" gene 1713..2522 /label=Kanamycin resistance gene /note="Kanamycin resistance gene" rep_origin 2643..3316 /label=f1 origin /note="f1 origin" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtatacaaa 661 agttgtgatg ggaaagtctt acccaactgt gagtgctgat taccaggacg ccgttgagaa 721 ggcgaagaag aagctcagag gcttcatcgc tgagaagaga tgcgctcctc taatgctccg 781 tttggcattc cactctgctg gaacctttga caagggcacg aagaccggtg gacccttcgg 841 aaccatcaag caccctgccg aactggctca cagcgctaac aacggtcttg acatcgctgt 901 taggcttttg gagccactca aggcggagtt ccctattttg agctacgccg atttctacca 961 gttggctggc gttgttgccg ttgaggtcac gggtggacct aaggttccat tccaccctgg 1021 aagagaggac aagcctgagc cgccaccaga gggtcgcttg cccgatccca ctaagggttc 1081 tgaccatttg agagatgtgt ttggcaaagc tatggggctt actgaccaag atatcgttgc 1141 tctatctggg ggtcacacta ttggagctgc acacaaggag cgttctggat ttgagggtcc 1201 ctggacctct aatcctctta ttttcgacaa ctcatacttc acggagttgt tgagtggtga 1261 gaaggaaggt ctccttcagc taccttctga caaggctctt ttgtctgacc ctgtattccg 1321 ccctctcgtt gacaaatatg cagcggacga agatgccttc tttgctgatt acgctgaggc 1381 tcaccaaaag ctttccgagc ttgggtttgc tgatgccggt ggaggcagcc acccaacttt 1441 tctatacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt 1501 cactatcagt caaaataaaa tcattatttg ccatccagct gcagggcggc cgcgatatcc 1561 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc 1621 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt 1681 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga 1741 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata 1801 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt 1861 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac 1921 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg 1981 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag 2041 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc 2101 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg 2161 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg 2221 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt 2281 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa 2341 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc 2401 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg 2461 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct 2521 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg 2581 gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag 2641 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 2701 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 2761 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 2821 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 2881 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 2941 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 3001 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 3061 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 3121 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 3181 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 3241 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 3301 ggccttttgc tcacatgtt //