LOCUS Exported 2989 bp ds-DNA circular SYN 17-JUL-2006 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pDONR221-P5P4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2989) AUTHORS Invitrogen TITLE Direct Submission JOURNAL Exported Jan 17, 2019 from SnapGene Viewer 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2989 /organism="synthetic DNA construct" /note="/dnas_title=Untitled Entry Clone Untitled Entry Clone Untitled L1-L4 L5-L4" misc_feature complement(268..295) /label=rrnB T2 transcription termination sequence /note="rrnB T2 transcription termination sequence" misc_feature complement(427..470) /label=rrnB T1 transcription termination sequence /note="rrnB T1 transcription termination sequence" primer_bind 517..533 /label=M13 Forward (-40) primer binding site /note="M13 Forward (-40) primer binding site" primer_bind 537..552 /label=M13 Forward (-20) primer binding site /note="M13 Forward (-20) primer binding site" misc_recomb 570..665 /label=attL5 /note="attL5 recombination site" /note="recognized as attL5" promoter 667..1100 /note="RIP" /note="Rat Insulin I promoter, pancreatic islet beta-cell specific" misc_recomb complement(1101..1199) /label=attL4 /note="attL4 recombination site" /note="recognized as attL4" misc_feature join(1111..2989,1..658) /label=nonstandard type: vector /note="/source=pDONR221-P5P4" /note="/type=Donor Vector" primer_bind complement(1254..1270) /label=M13 Reverse primer binding site /note="M13 Reverse primer binding site" gene 1383..2192 /label=Kanamycin resistance gene /note="Kanamycin resistance gene" rep_origin 2313..2986 /label=f1 origin /note="f1 origin" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtatacaaa 661 agttgtaaga ctgagctaag aatccagcta tcaatagaaa ctatgaaaca gttccaggga 721 caaagatacc aggtccccaa caactgcaac tttctgggaa atgaggtgga aaatgctcag 781 ccaaggaaaa agagggcctt accctctctg ggacaatgat tgtgctgtga actgcttcat 841 caggccatct ggccccttgt taataatcta attaccctag gtctaagtag agttgttgac 901 gtccaatgag cgctttctgc agacttagca ctaggcaagt gtttggaaat tacagcttca 961 gcccctctcg ccatctgcct acctacccct cctagagccc ttaatgggcc aaacggcaaa 1021 gtccaggggg cagagaggag gtgctttgga ctataaagct agtggagacc cagtaactcc 1081 caaccctaag tgaccagctc acccaacttt tctatacaaa gttggcatta taagaaagca 1141 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg 1201 ccatccagct gcagggcggc cgcgatatcc cctatagtga gtcgtattac atggtcatag 1261 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata 1321 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg 1381 ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg 1441 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc 1501 gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg 1561 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc 1621 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc 1681 ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg 1741 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta 1801 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg 1861 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa 1921 tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg 1981 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa 2041 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt 2101 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc 2161 agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact 2221 ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg 2281 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 2341 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 2401 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 2461 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 2521 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 2581 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 2641 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 2701 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 2761 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 2821 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 2881 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 2941 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtt //