LOCUS       Exported                2989 bp ds-DNA     circular SYN 17-JUL-2006
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDONR221-P5P4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Invitrogen
  TITLE     Direct Submission
  JOURNAL   Exported Jan 17, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=Untitled Entry Clone Untitled Entry 
                     Clone Untitled L1-L4 L5-L4"
     misc_feature    complement(268..295)
                     /label=rrnB T2 transcription termination sequence
                     /note="rrnB T2 transcription termination sequence"
     misc_feature    complement(427..470)
                     /label=rrnB T1 transcription termination sequence
                     /note="rrnB T1 transcription termination sequence"
     primer_bind     517..533
                     /label=M13 Forward (-40) primer binding site
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     537..552
                     /label=M13 Forward (-20) primer binding site
                     /note="M13 Forward (-20) primer binding site"
     misc_recomb     570..665
                     /label=attL5
                     /note="attL5 recombination site"
                     /note="recognized as attL5"
     promoter        667..1100
                     /note="RIP"
                     /note="Rat Insulin I promoter, pancreatic islet beta-cell 
                     specific"
     misc_recomb     complement(1101..1199)
                     /label=attL4
                     /note="attL4 recombination site"
                     /note="recognized as attL4"
     misc_feature    join(1111..2989,1..658)
                     /label=nonstandard type: vector
                     /note="/source=pDONR221-P5P4"
                     /note="/type=Donor Vector"
     primer_bind     complement(1254..1270)
                     /label=M13 Reverse primer binding site
                     /note="M13 Reverse primer binding site"
     gene            1383..2192
                     /label=Kanamycin resistance gene
                     /note="Kanamycin resistance gene"
     rep_origin      2313..2986
                     /label=f1 origin
                     /note="f1 origin"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtatacaaa
      661 agttgtaaga ctgagctaag aatccagcta tcaatagaaa ctatgaaaca gttccaggga
      721 caaagatacc aggtccccaa caactgcaac tttctgggaa atgaggtgga aaatgctcag
      781 ccaaggaaaa agagggcctt accctctctg ggacaatgat tgtgctgtga actgcttcat
      841 caggccatct ggccccttgt taataatcta attaccctag gtctaagtag agttgttgac
      901 gtccaatgag cgctttctgc agacttagca ctaggcaagt gtttggaaat tacagcttca
      961 gcccctctcg ccatctgcct acctacccct cctagagccc ttaatgggcc aaacggcaaa
     1021 gtccaggggg cagagaggag gtgctttgga ctataaagct agtggagacc cagtaactcc
     1081 caaccctaag tgaccagctc acccaacttt tctatacaaa gttggcatta taagaaagca
     1141 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     1201 ccatccagct gcagggcggc cgcgatatcc cctatagtga gtcgtattac atggtcatag
     1261 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
     1321 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
     1381 ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg
     1441 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
     1501 gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
     1561 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc
     1621 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
     1681 ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
     1741 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
     1801 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
     1861 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
     1921 tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
     1981 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa
     2041 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
     2101 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
     2161 agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact
     2221 ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg
     2281 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2341 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2401 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2461 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2521 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2581 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2641 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2701 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2761 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2821 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2881 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2941 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtt
//
