LOCUS       Exported                2890 bp ds-DNA     circular SYN 17-JUL-2006
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDONR221-P5P4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Invitrogen
  TITLE     Direct Submission
  JOURNAL   Exported Jan 17, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=Untitled Entry Clone GZ801 L5-L4"
     misc_feature    complement(268..295)
                     /label=rrnB T2 transcription termination sequence
                     /note="rrnB T2 transcription termination sequence"
     misc_feature    complement(427..470)
                     /label=rrnB T1 transcription termination sequence
                     /note="rrnB T1 transcription termination sequence"
     primer_bind     517..533
                     /label=M13 Forward (-40) primer binding site
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     537..552
                     /label=M13 Forward (-20) primer binding site
                     /note="M13 Forward (-20) primer binding site"
     misc_recomb     570..665
                     /label=attL5
                     /note="attL5 recombination site"
                     /note="recognized as attL5"
     promoter        670..990
                     /note="TRE promoter"
                     /note="tet-responsive promoter"
     misc_feature    679..697
                     /label=tetO 19mer #1
                     /note="tetO 19mer #1"
     misc_feature    715..733
                     /label=tetO 19mer #2
                     /note="tetO 19mer #2"
     misc_feature    750..768
                     /label=tetO 19mer #3
                     /note="tetO 19mer #3"
     misc_feature    786..804
                     /label=tetO 19mer #4
                     /note="tetO 19mer #4"
     misc_feature    822..840
                     /label=tetO 19mer #5
                     /note="tetO 19mer #5"
     misc_feature    857..875
                     /label=tetO 19mer #6
                     /note="tetO 19mer #6"
     misc_feature    893..911
                     /label=tetO 19mer #7
                     /note="tetO 19mer #7"
     promoter        923..982
                     /note="minimal CMV promoter"
     misc_feature    976..990
                     /label=CMV exon 1
                     /note="CMV exon 1"
     misc_recomb     complement(1001..1100)
                     /label=attL4
                     /note="attL4 recombination site"
                     /note="recognized as attL4"
     misc_feature    join(1012..2890,1..658)
                     /label=nonstandard type: vector
                     /note="/source=pDONR221-P5P4"
                     /note="/type=Donor Vector"
     primer_bind     complement(1155..1171)
                     /label=M13 Reverse primer binding site
                     /note="M13 Reverse primer binding site"
     gene            1284..2093
                     /label=Kanamycin resistance gene
                     /note="Kanamycin resistance gene"
     rep_origin      2214..2887
                     /label=f1 origin
                     /note="f1 origin"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtatacaaa
      661 agttggcctc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta
      721 tcagtgatag agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag
      781 tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
      841 acgtatgtcg agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc
      901 agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc
      961 tcgtttagtg aaccgtcaga tcgcctggag aattggatcc aacccaactt ttctatacaa
     1021 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
     1081 tcaaaataaa atcattattt gccatccagc tgcagggcgg ccgcgatatc ccctatagtg
     1141 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     1201 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
     1261 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat
     1321 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     1381 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga
     1441 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
     1501 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     1561 ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg
     1621 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
     1681 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
     1741 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
     1801 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca
     1861 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
     1921 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
     1981 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     2041 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat
     2101 tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct
     2161 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     2221 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2281 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2341 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2401 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2461 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2521 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2581 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     2641 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     2701 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     2761 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     2821 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     2881 ctcacatgtt
//
