LOCUS       Exported                3378 bp ds-DNA     circular SYN 17-JUL-2006
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDONR221-P4rP3r
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Invitrogen
  TITLE     Direct Submission
  JOURNAL   Exported Jan 17, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=IO201_mCherry"
     misc_feature    complement(268..295)
                     /label=rrnB T2 transcription termination sequence
                     /note="rrnB T2 transcription termination sequence"
     misc_feature    complement(427..470)
                     /label=rrnB T1 transcription termination sequence
                     /note="rrnB T1 transcription termination sequence"
     primer_bind     517..533
                     /label=M13 Forward (-40) primer binding site
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     537..552
                     /label=M13 Forward (-20) primer binding site
                     /note="M13 Forward (-20) primer binding site"
     misc_recomb     complement(570..726)
                     /label=attL4r
                     /note="attL4r recombination site"
                     /note="recognized as attR4"
     gene            729..1439
                     /label=mCherry
                     /note="mCherry"
     misc_recomb     1440..1597
                     /label=attL3r
                     /note="attL3r recombination site"
                     /note="recognized as attR3"
     misc_feature    join(1448..3378,1..719)
                     /label=nonstandard type: vector
                     /note="/source=pDONR221-P4rP3r"
                     /note="/type=Donor Vector"
     primer_bind     complement(1643..1659)
                     /label=M13 Reverse primer binding site
                     /note="M13 Reverse primer binding site"
     gene            1772..2581
                     /label=Kanamycin resistance gene
                     /note="Kanamycin resistance gene"
     rep_origin      2702..3375
                     /label=f1 origin
                     /note="f1 origin"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccct acaggtcact aataccatct aagtagttga
      601 ttcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
      661 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact tttctataca
      721 aagttgccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
      781 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
      841 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
      901 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
      961 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     1021 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     1081 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     1141 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     1201 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     1261 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     1321 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     1381 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaaa
     1441 caactttgta taataaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     1501 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     1561 tatgaatcaa ctacttagat ggtattagtg acctgtactg cagggcggcc gcgatatccc
     1621 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     1681 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     1741 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     1801 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     1861 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     1921 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     1981 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     2041 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     2101 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     2161 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     2221 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     2281 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     2341 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     2401 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     2461 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     2521 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     2581 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     2641 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     2701 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2761 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2821 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2881 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2941 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3001 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3061 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3121 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3181 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3241 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3301 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3361 gccttttgct cacatgtt
//
