LOCUS Exported 3378 bp ds-DNA circular SYN 17-JUL-2006 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pDONR221-P4rP3r SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3378) AUTHORS Invitrogen TITLE Direct Submission JOURNAL Exported Jan 17, 2019 from SnapGene Viewer 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3378 /organism="synthetic DNA construct" /note="/dnas_title=Untitled Entry Clone mCherry L4-L3" misc_feature complement(268..295) /label=rrnB T2 transcription termination sequence /note="rrnB T2 transcription termination sequence" misc_feature complement(427..470) /label=rrnB T1 transcription termination sequence /note="rrnB T1 transcription termination sequence" primer_bind 517..533 /label=M13 Forward (-40) primer binding site /note="M13 Forward (-40) primer binding site" primer_bind 537..552 /label=M13 Forward (-20) primer binding site /note="M13 Forward (-20) primer binding site" misc_recomb complement(570..727) /label=attL4r /note="attL4r recombination site" /note="recognized as attR4" gene 732..1439 /label=mCherry (open) /note="mCherry (open)" misc_recomb 1440..1597 /label=attL3r /note="attL3r recombination site" /note="recognized as attR3" misc_feature join(1448..3378,1..719) /label=nonstandard type: vector /note="/source=pDONR221-P4rP3r" /note="/type=Donor Vector" primer_bind complement(1643..1659) /label=M13 Reverse primer binding site /note="M13 Reverse primer binding site" gene 1772..2581 /label=Kanamycin resistance gene /note="Kanamycin resistance gene" rep_origin 2702..3375 /label=f1 origin /note="f1 origin" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccct acaggtcact aataccatct aagtagttga 601 ttcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa 661 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact tttctataca 721 aagttgttac catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca 781 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg 841 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg 901 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct 961 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca 1021 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct 1081 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg 1141 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc 1201 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc 1261 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg 1321 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg 1381 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaaga 1441 caactttgta taataaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa 1501 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac 1561 tatgaatcaa ctacttagat ggtattagtg acctgtactg cagggcggcc gcgatatccc 1621 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca 1681 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc 1741 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag 1801 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa 1861 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt 1921 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact 1981 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga 2041 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga 2101 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca 2161 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc 2221 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg 2281 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc 2341 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat 2401 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct 2461 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg 2521 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta 2581 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg 2641 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga 2701 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 2761 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 2821 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct 2881 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 2941 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 3001 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 3061 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 3121 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 3181 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 3241 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 3301 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 3361 gccttttgct cacatgtt //