LOCUS       Exported                3151 bp ds-DNA     circular SYN 17-JUL-2006
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDONR221-P3P2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Invitrogen
  TITLE     Direct Submission
  JOURNAL   Exported Jan 17, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=HB901_hGH_polyA"
     misc_feature    complement(268..295)
                     /label=rrnB T2 transcription termination sequence
                     /note="rrnB T2 transcription termination sequence"
     misc_feature    complement(427..470)
                     /label=rrnB T1 transcription termination sequence
                     /note="rrnB T1 transcription termination sequence"
     primer_bind     517..533
                     /label=M13 Forward (-40) primer binding site
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     537..552
                     /label=M13 Forward (-20) primer binding site
                     /note="M13 Forward (-20) primer binding site"
     misc_recomb     570..666
                     /label=attL3
                     /note="attL3 recombination site"
                     /note="recognized as attL3"
     polyA_signal    667..1261
                     /label=hGH PolyA signal
                     /note="hGH PolyA signal"
                     /note="human growth hormone polyadenylation signal"
     misc_recomb     complement(1262..1361)
                     /label=attL2
                     /note="attL2 recombination site"
                     /note="recognized as attL2"
     misc_feature    join(1273..3151,1..658)
                     /label=nonstandard type: vector
                     /note="/source=pDONR221-P3P2"
                     /note="/type=Donor Vector"
     primer_bind     complement(1416..1432)
                     /label=M13 Reverse primer binding site
                     /note="M13 Reverse primer binding site"
     gene            1545..2354
                     /label=Kanamycin resistance gene
                     /note="Kanamycin resistance gene"
     rep_origin      2475..3148
                     /label=f1 origin
                     /note="f1 origin"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataataa
      661 agttgtacag tgcctctcct ggccttggaa gttgccactc cagtgcccac cagccttgtc
      721 ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg
      781 tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg
      841 gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc
      901 tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc
      961 atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc
     1021 aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg
     1081 ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttaaa ataactatac
     1141 cagcaggagg acgtccagac acagcatagg ctacctggcc atgcccaacc ggtgggacat
     1201 ttgagttgct tgcttggcac tgtcctctca tgcgttgggt ccactcagta gatgcctgtt
     1261 gaacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     1321 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgcagggcg
     1381 gccgcgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     1441 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
     1501 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
     1561 gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     1621 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg
     1681 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     1741 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
     1801 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc
     1861 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
     1921 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     1981 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     2041 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat
     2101 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     2161 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     2221 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     2281 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     2341 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga
     2401 cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     2461 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2521 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2581 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2641 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2701 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2761 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2821 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2881 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2941 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3001 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3061 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3121 tggccttttg ctggcctttt gctcacatgt t
//
