LOCUS       Exported                2856 bp ds-DNA     circular SYN 17-JUL-2006
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDONR221-P3P2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Invitrogen
  TITLE     Direct Submission
  JOURNAL   Exported Jan 17, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=KG801_V5_bGHpolyA"
     misc_feature    complement(268..295)
                     /label=rrnB T2 transcription termination sequence
                     /note="rrnB T2 transcription termination sequence"
     misc_feature    complement(427..470)
                     /label=rrnB T1 transcription termination sequence
                     /note="rrnB T1 transcription termination sequence"
     primer_bind     517..533
                     /label=M13 Forward (-40) primer binding site
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     537..552
                     /label=M13 Forward (-20) primer binding site
                     /note="M13 Forward (-20) primer binding site"
     misc_recomb     570..667
                     /label=attL3
                     /note="attL3 recombination site"
                     /note="recognized as attL3"
     misc_feature    665..676
                     /label=GGGS linker
                     /note="GGGS linker"
     misc_feature    677..721
                     /label=V5 epitope tag
                     /note="V5 epitope tag"
     polyA_signal    749..959
                     /label=bGH Poly A
                     /note="bGH Poly A"
                     /note="shorter sequence than in pEF-DEST51 (invitrogen)"
     misc_recomb     complement(969..1066)
                     /label=attL2
                     /note="attL2 recombination site"
                     /note="recognized as attL2"
     misc_feature    join(978..2856,1..658)
                     /label=nonstandard type: vector
                     /note="/source=pDONR221-P3P2"
                     /note="/type=Donor Vector"
     primer_bind     complement(1121..1137)
                     /label=M13 Reverse primer binding site
                     /note="M13 Reverse primer binding site"
     gene            1250..2059
                     /label=Kanamycin resistance gene
                     /note="Kanamycin resistance gene"
     rep_origin      2180..2853
                     /label=f1 origin
                     /note="f1 origin"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataataa
      661 agttggtggc ggatccggta agcctatccc taaccctctc ctcggtctcg attctacgta
      721 agaattccta gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
      781 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
      841 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
      901 ggggtggggc aggacagcaa gggggaggat tgggaagaga atagcaggca tgctggggag
      961 gtacctgacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     1021 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgca
     1081 gggcggccgc gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     1141 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     1201 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     1261 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     1321 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
     1381 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     1441 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     1501 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     1561 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     1621 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     1681 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     1741 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     1801 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     1861 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     1921 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     1981 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     2041 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac
     2101 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc
     2161 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2221 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2281 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2341 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2401 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2461 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2521 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2581 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2641 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2701 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2761 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2821 gttcctggcc ttttgctggc cttttgctca catgtt
//
