LOCUS       Exported                3256 bp ds-DNA     circular SYN 17-JUL-2006
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDONR221-P3P2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Invitrogen
  TITLE     Direct Submission
  JOURNAL   Exported Jan 17, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=KH101_HA_WPRE"
     misc_feature    complement(268..295)
                     /label=rrnB T2 transcription termination sequence
                     /note="rrnB T2 transcription termination sequence"
     misc_feature    complement(427..470)
                     /label=rrnB T1 transcription termination sequence
                     /note="rrnB T1 transcription termination sequence"
     primer_bind     517..533
                     /label=M13 Forward (-40) primer binding site
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     537..552
                     /label=M13 Forward (-20) primer binding site
                     /note="M13 Forward (-20) primer binding site"
     misc_recomb     570..667
                     /label=attL3
                     /note="attL3 recombination site"
                     /note="recognized as attL3"
     misc_feature    665..676
                     /label=GGGS linker
                     /note="GGGS linker"
     misc_feature    677..706
                     /label=HA epitope tag
                     /note="HA epitope tag"
     terminator      747..1344
                     /note="WPRE"
     misc_recomb     complement(1369..1466)
                     /label=attL2
                     /note="attL2 recombination site"
                     /note="recognized as attL2"
     misc_feature    join(1378..3256,1..658)
                     /label=nonstandard type: vector
                     /note="/source=pDONR221-P3P2"
                     /note="/type=Donor Vector"
     primer_bind     complement(1521..1537)
                     /label=M13 Reverse primer binding site
                     /note="M13 Reverse primer binding site"
     gene            1650..2459
                     /label=Kanamycin resistance gene
                     /note="Kanamycin resistance gene"
     rep_origin      2580..3253
                     /label=f1 origin
                     /note="f1 origin"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataataa
      661 agttggtggc ggatcctacc catacgatgt gccagattac gcttaagaat tcctagagct
      721 cctatagtga gaccggttag taatgatcga caatcaacct ctggattaca aaatttgtga
      781 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
      841 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
      901 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
      961 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     1021 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     1081 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     1141 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     1201 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     1261 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     1321 cctttgggcc gcctccccgc ctggcgatgg tactgtccta gtacctgacc cagctttctt
     1381 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     1441 tatcagtcaa aataaaatca ttatttgcca tccagctgca gggcggccgc gatatcccct
     1501 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     1561 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg
     1621 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc
     1681 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     1741 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt
     1801 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     1861 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     1921 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat
     1981 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     2041 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     2101 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     2161 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag
     2221 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     2281 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     2341 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     2401 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     2461 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg
     2521 caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     2581 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2641 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2701 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2761 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2821 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2881 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2941 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3001 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3061 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3121 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3181 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3241 cttttgctca catgtt
//
