LOCUS       Exported                3310 bp ds-DNA     circular SYN 17-JUL-2006
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDONR221-P3P2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Invitrogen
  TITLE     Direct Submission
  JOURNAL   Exported Jan 17, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=IX901_3xHA_WPRE"
     misc_feature    complement(268..295)
                     /label=rrnB T2 transcription termination sequence
                     /note="rrnB T2 transcription termination sequence"
     misc_feature    complement(427..470)
                     /label=rrnB T1 transcription termination sequence
                     /note="rrnB T1 transcription termination sequence"
     primer_bind     517..533
                     /label=M13 Forward (-40) primer binding site
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     537..552
                     /label=M13 Forward (-20) primer binding site
                     /note="M13 Forward (-20) primer binding site"
     misc_recomb     570..667
                     /label=attL3
                     /note="attL3 recombination site"
                     /note="recognized as attL3"
     misc_feature    665..676
                     /label=GGGS linker
                     /note="GGGS linker"
     gene            677..761
                     /label=3x HA epitope tag
                     /note="3x HA epitope tag"
     terminator      801..1398
                     /note="WPRE"
     misc_recomb     complement(1423..1520)
                     /label=attL2
                     /note="attL2 recombination site"
                     /note="recognized as attL2"
     misc_feature    join(1432..3310,1..658)
                     /label=nonstandard type: vector
                     /note="/source=pDONR221-P3P2"
                     /note="/type=Donor Vector"
     primer_bind     complement(1575..1591)
                     /label=M13 Reverse primer binding site
                     /note="M13 Reverse primer binding site"
     gene            1704..2513
                     /label=Kanamycin resistance gene
                     /note="Kanamycin resistance gene"
     rep_origin      2634..3307
                     /label=f1 origin
                     /note="f1 origin"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataataa
      661 agttggtggc ggatcctacc catacgatgt tccagattac gcttacccat acgatgttcc
      721 agattacgct tacccatacg atgttccaga ttacgcttaa gaattcctag agctcctata
      781 gtgagaccgg ttagtaatga tcgacaatca acctctggat tacaaaattt gtgaaagatt
      841 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
      901 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
      961 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     1021 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     1081 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     1141 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     1201 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     1261 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     1321 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     1381 ggccgcctcc ccgcctggcg atggtactgt cctagtacct gacccagctt tcttgtacaa
     1441 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
     1501 tcaaaataaa atcattattt gccatccagc tgcagggcgg ccgcgatatc ccctatagtg
     1561 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     1621 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
     1681 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat
     1741 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     1801 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga
     1861 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
     1921 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     1981 ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg
     2041 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
     2101 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
     2161 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
     2221 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca
     2281 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
     2341 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
     2401 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     2461 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat
     2521 tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct
     2581 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     2641 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2701 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2761 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2821 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2881 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2941 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3001 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3061 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3121 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3181 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3241 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3301 ctcacatgtt
//
