LOCUS Exported 3247 bp ds-DNA circular SYN 17-JUL-2006 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pDONR221-P3P2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3247) AUTHORS Invitrogen TITLE Direct Submission JOURNAL Exported Jan 17, 2019 from SnapGene Viewer 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3247 /organism="synthetic DNA construct" /note="/dnas_title=IZ801_6xHis_tag_WPRE" misc_feature complement(268..295) /label=rrnB T2 transcription termination sequence /note="rrnB T2 transcription termination sequence" misc_feature complement(427..470) /label=rrnB T1 transcription termination sequence /note="rrnB T1 transcription termination sequence" primer_bind 517..533 /label=M13 Forward (-40) primer binding site /note="M13 Forward (-40) primer binding site" primer_bind 537..552 /label=M13 Forward (-20) primer binding site /note="M13 Forward (-20) primer binding site" misc_recomb 570..667 /label=attL3 /note="attL3 recombination site" /note="recognized as attL3" misc_feature 665..676 /label=GGGS linker /note="GGGS linker" gene 677..694 /label=6x His epitope tag /note="6x His epitope tag" terminator 738..1335 /note="WPRE" misc_recomb complement(1360..1457) /label=attL2 /note="attL2 recombination site" /note="recognized as attL2" misc_feature join(1369..3247,1..658) /label=nonstandard type: vector /note="/source=pDONR221-P3P2" /note="/type=Donor Vector" primer_bind complement(1512..1528) /label=M13 Reverse primer binding site /note="M13 Reverse primer binding site" gene 1641..2450 /label=Kanamycin resistance gene /note="Kanamycin resistance gene" rep_origin 2571..3244 /label=f1 origin /note="f1 origin" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataataa 661 agttggtggc ggatcccacc atcatcacca tcactaagaa ttcctagagc tcctatagtg 721 agaccggtta gtaatgatcg acaatcaacc tctggattac aaaatttgtg aaagattgac 781 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 841 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 901 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 961 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 1021 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 1081 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct 1141 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 1201 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 1261 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc 1321 cgcctccccg cctggcgatg gtactgtcct agtacctgac ccagctttct tgtacaaagt 1381 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca 1441 aaataaaatc attatttgcc atccagctgc agggcggccg cgatatcccc tatagtgagt 1501 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat 1561 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa 1621 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa 1681 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat 1741 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac 1801 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga 1861 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt 1921 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt 1981 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg 2041 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa 2101 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg 2161 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc 2221 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg 2281 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc 2341 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc 2401 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg 2461 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat 2521 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa 2581 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 2641 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 2701 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 2761 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 2821 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 2881 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 2941 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 3001 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 3061 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 3121 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 3181 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 3241 acatgtt //