LOCUS pDONR221-P3P2 4774 bp DNA circular SYN 23-APR-2009 SOURCE ORGANISM COMMENT VNTDATE|391339286| COMMENT VNTDBDATE|416244976| COMMENT VNTNAME|pDONR221-P3P2| COMMENT VNTAUTHORNAME|Invitrogen| COMMENT VNTAUTHORTEL|(800)-357-3114| COMMENT VNTAUTHORFAX|(760)-602-6500| COMMENT VNTOAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com| COMMENT VNTAUTHOREML|bioinfosales@invitrogen.com| COMMENT VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics| COMMENT VNTAUTHORAD1|Invitrogen Corporation| COMMENT VNTAUTHORAD2|1620 Faraday Avenue| COMMENT VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.| FEATURES Location/Qualifiers misc_recomb 570..667 /label=attL3 /note="attL3 recombination site" /note="recognized as attL3" misc_recomb complement(1658..1755) /label=attL2 /note="attL2 recombination site" /note="recognized as attL2" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence" misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence" primer_bind 537..552 /note="M13 Forward (-20) primer binding site" primer_bind 517..533 /note="M13 Forward (-40) primer binding site" primer_bind complement(1810..1826) /note="M13 Reverse primer binding site" rep_origin 2869..3542 /note="f1 origin" gene 1939..2748 /note="Kanamycin resistance gene" polyA_signal 1438..1648 /note="bGH Poly A " /note="bovine growth hormone polyadenylation signal" vector join(1667..3545,1..658) /source="pDONR221-P3P2" /type="Donor Vector" gene 680..1399 /note="eGFP" misc_feature 665..679 /note="GGGGS linker" source 1..3545 /dnas_title="LC401_GFP_polyA" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataatAA 661 AGTTGGTGGC GGcGGAtccA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC 721 CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG 781 CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT 841 GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG 901 CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT 961 CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA 1021 GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA 1081 CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT 1141 GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA 1201 CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT 1261 GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA 1321 GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT 1381 GGACGAGCTG TACAAGtaag aattctgaag cttagcctag agctcgctga tcagcctcga 1441 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc 1501 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc 1561 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt 1621 gggaagagaa tagcaggcat gctggggagg tacctgaCCC AGCTTTcttg tacaaagttg 1681 gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa 1741 ataaaatcat tatttgccat ccagctgcag ggcggccgcg atatccccta tagtgagtcg 1801 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt 1861 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac 1921 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat 1981 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca 2041 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat 2101 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg 2161 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta 2221 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca 2281 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt 2341 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg 2401 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa 2461 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat 2521 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat 2581 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc 2641 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct 2701 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt 2761 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga 2821 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag 2881 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 2941 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 3001 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag 3061 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 3121 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 3181 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 3241 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 3301 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 3361 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 3421 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 3481 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 3541 atgtt //