LOCUS pDONR221-P3P2 4774 bp DNA circular SYN 23-APR-2009 SOURCE ORGANISM COMMENT VNTDATE|391339286| COMMENT VNTDBDATE|416244976| COMMENT VNTNAME|pDONR221-P3P2| COMMENT VNTAUTHORNAME|Invitrogen| COMMENT VNTAUTHORTEL|(800)-357-3114| COMMENT VNTAUTHORFAX|(760)-602-6500| COMMENT VNTOAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com| COMMENT VNTAUTHOREML|bioinfosales@invitrogen.com| COMMENT VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics| COMMENT VNTAUTHORAD1|Invitrogen Corporation| COMMENT VNTAUTHORAD2|1620 Faraday Avenue| COMMENT VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.| FEATURES Location/Qualifiers misc_recomb 570..667 /label=attL3 /note="attL3 recombination site" /note="recognized as attL3" misc_recomb complement(1721..1818) /label=attL2 /note="attL2 recombination site" /note="recognized as attL2" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence" misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence" primer_bind 537..552 /note="M13 Forward (-20) primer binding site" primer_bind 517..533 /note="M13 Forward (-40) primer binding site" primer_bind complement(1873..1889) /note="M13 Reverse primer binding site" rep_origin 2932..3605 /note="f1 origin" gene 2002..2811 /note="Kanamycin resistance gene" polyA_signal 1501..1711 /note="bGH Poly A " /note="bovine growth hormone polyadenylation signal" vector join(1730..3608,1..658) /source="pDONR221-P3P2" /type="Donor Vector" misc_feature 671..679 /note="GSG linker" misc_feature 680..736 /note="P2A self-cleaving peptide" misc_feature 731..736 /note="G^P cleavage site" gene 743..1462 /note="eGFP" source 1..3608 /dnas_title="KY801_P2A_GFP_polyA" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataatAA 661 AGTTGGTGGC GGATCcGGAG CTACTAATTT CTCTCTGTTA AAGCAGGCAG GAGATGTGGA 721 GGAGAATCCA GGTCCTAAGG GAATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT 781 GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA 841 GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA 901 GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG 961 CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA 1021 CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT 1081 GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA 1141 GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT 1201 CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA 1261 GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC 1321 CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA 1381 CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG 1441 CATGGACGAG CTGTACAAGt aagaattctg aagcttagcc tagagctcgc tgatcagcct 1501 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 1561 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 1621 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 1681 attgggaaga gaatagcagg catgctgggg aggtacctga CCCAGCTTTc ttgtacaaag 1741 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc 1801 aaaataaaat cattatttgc catccagctg cagggcggcc gcgatatccc ctatagtgag 1861 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga 1921 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata 1981 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta 2041 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa 2101 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa 2161 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg 2221 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg 2281 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat 2341 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct 2401 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga 2461 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt 2521 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact 2581 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt 2641 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc 2701 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat 2761 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg 2821 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca 2881 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa 2941 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 3001 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 3061 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 3121 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 3181 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 3241 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 3301 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 3361 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 3421 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 3481 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 3541 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 3601 cacatgtt //