LOCUS       pDONR221-P3P2           4774 bp    DNA     circular SYN 23-APR-2009
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|391339286|
COMMENT     VNTDBDATE|416244976|
COMMENT     VNTNAME|pDONR221-P3P2|
COMMENT     VNTAUTHORNAME|Invitrogen|
COMMENT     VNTAUTHORTEL|(800)-357-3114|
COMMENT     VNTAUTHORFAX|(760)-602-6500|
COMMENT     VNTOAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com|
COMMENT     VNTAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics|
COMMENT     VNTAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
FEATURES             Location/Qualifiers
     misc_recomb     570..667
                     /label=attL3
                     /note="attL3 recombination site"
                     /note="recognized as attL3"
     misc_recomb     complement(1649..1746)
                     /label=attL2
                     /note="attL2 recombination site"
                     /note="recognized as attL2"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence"
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence"
     primer_bind     537..552
                     /note="M13 Forward (-20) primer binding site"
     primer_bind     517..533
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     complement(1801..1817)
                     /note="M13 Reverse primer binding site"
     rep_origin      2860..3533
                     /note="f1 origin"
     gene            1930..2739
                     /note="Kanamycin resistance gene"
     polyA_signal    1429..1639
                     /note="bGH Poly A "
                     /note="bovine growth hormone polyadenylation signal"
     vector          join(1658..3536,1..658)
                     /source="pDONR221-P3P2"
                     /type="Donor Vector"
     gene            680..1390
                     /note="mCherry"
     misc_feature    665..679
                     /note="GGGGS linker"
     source          1..3536
                     /dnas_title="LC501_mCherry_polyA"
ORIGIN      
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataatAA
      661 AGTTGGTGGC GGcGGAtcca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
      721 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
      781 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
      841 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
      901 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
      961 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     1021 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     1081 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     1141 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     1201 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     1261 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     1321 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     1381 gtacaagtaa gaattctgaa gcttagccta gagctcgctg atcagcctcg actgtgcctt
     1441 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     1501 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     1561 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaga
     1621 atagcaggca tgctggggag gtacctgaCC CAGCTTTctt gtacaaagtt ggcattataa
     1681 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     1741 ttatttgcca tccagctgca gggcggccgc gatatcccct atagtgagtc gtattacatg
     1801 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     1861 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     1921 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     1981 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     2041 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     2101 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     2161 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     2221 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     2281 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     2341 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     2401 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     2461 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     2521 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     2581 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     2641 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     2701 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     2761 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
     2821 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2881 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2941 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3001 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3061 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3121 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3181 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3241 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3301 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3361 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3421 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3481 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgtt
//

