LOCUS pDONR221-P3P2 4774 bp DNA circular SYN 23-APR-2009 SOURCE ORGANISM COMMENT VNTDATE|391339286| COMMENT VNTDBDATE|416244976| COMMENT VNTNAME|pDONR221-P3P2| COMMENT VNTAUTHORNAME|Invitrogen| COMMENT VNTAUTHORTEL|(800)-357-3114| COMMENT VNTAUTHORFAX|(760)-602-6500| COMMENT VNTOAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com| COMMENT VNTAUTHOREML|bioinfosales@invitrogen.com| COMMENT VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics| COMMENT VNTAUTHORAD1|Invitrogen Corporation| COMMENT VNTAUTHORAD2|1620 Faraday Avenue| COMMENT VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.| FEATURES Location/Qualifiers misc_recomb 570..667 /label=attL3 /note="attL3 recombination site" /note="recognized as attL3" misc_recomb complement(1712..1809) /label=attL2 /note="attL2 recombination site" /note="recognized as attL2" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence" misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence" primer_bind 537..552 /note="M13 Forward (-20) primer binding site" primer_bind 517..533 /note="M13 Forward (-40) primer binding site" primer_bind complement(1864..1880) /note="M13 Reverse primer binding site" rep_origin 2923..3596 /note="f1 origin" gene 1993..2802 /note="Kanamycin resistance gene" polyA_signal 1492..1702 /note="bGH Poly A " /note="bovine growth hormone polyadenylation signal" vector join(1721..3599,1..658) /source="pDONR221-P3P2" /type="Donor Vector" misc_feature 671..679 /note="GSG linker" misc_feature 680..736 /note="P2A self-cleaving peptide" misc_feature 731..736 /note="G^P cleavage site" gene 743..1453 /note="mCherry" source 1..3599 /dnas_title="KY901_P2A_mCherry_polyA" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataatAA 661 AGTTGGTGGC GGATCcGGAG CTACTAATTT CTCTCTGTTA AAGCAGGCAG GAGATGTGGA 721 GGAGAATCCA GGTCCTAAGG GAatggtgag caagggcgag gaggataaca tggccatcat 781 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga 841 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt 901 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg 961 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc 1021 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac 1081 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa 1141 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga 1201 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa 1261 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca 1321 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta 1381 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga 1441 gctgtacaag taagaattct gaagcttagc ctagagctcg ctgatcagcc tcgactgtgc 1501 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag 1561 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta 1621 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag 1681 agaatagcag gcatgctggg gaggtacctg aCCCAGCTTT cttgtacaaa gttggcatta 1741 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa 1801 tcattatttg ccatccagct gcagggcggc cgcgatatcc cctatagtga gtcgtattac 1861 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt 1921 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat 1981 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac 2041 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg 2101 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa 2161 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt 2221 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc 2281 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa 2341 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat 2401 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac 2461 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc 2521 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat 2581 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga 2641 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag 2701 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg 2761 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg 2821 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa 2881 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa 2941 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 3001 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 3061 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc 3121 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 3181 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 3241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 3301 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 3361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 3421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 3481 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 3541 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtt //