LOCUS       pDONR221-P3P2           4774 bp    DNA     circular SYN 23-APR-2009
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|391339286|
COMMENT     VNTDBDATE|416244976|
COMMENT     VNTNAME|pDONR221-P3P2|
COMMENT     VNTAUTHORNAME|Invitrogen|
COMMENT     VNTAUTHORTEL|(800)-357-3114|
COMMENT     VNTAUTHORFAX|(760)-602-6500|
COMMENT     VNTOAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com|
COMMENT     VNTAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics|
COMMENT     VNTAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
FEATURES             Location/Qualifiers
     misc_recomb     570..667
                     /label=attL3
                     /note="attL3 recombination site"
                     /note="recognized as attL3"
     misc_recomb     complement(1721..1818)
                     /label=attL2
                     /note="attL2 recombination site"
                     /note="recognized as attL2"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence"
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence"
     primer_bind     537..552
                     /note="M13 Forward (-20) primer binding site"
     primer_bind     517..533
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     complement(1873..1889)
                     /note="M13 Reverse primer binding site"
     rep_origin      2932..3605
                     /note="f1 origin"
     gene            2002..2811
                     /note="Kanamycin resistance gene"
     polyA_signal    1501..1711
                     /note="bGH Poly A "
                     /note="bovine growth hormone polyadenylation signal"
     vector          join(1730..3608,1..658)
                     /source="pDONR221-P3P2"
                     /type="Donor Vector"
     misc_feature    671..679
                     /note="GSG linker"
     misc_feature    680..736
                     /note="P2A self-cleaving peptide"
     misc_feature    731..736
                     /note="G^P cleavage site"
     gene            743..1462
                     /note="eCFP"
     source          1..3608
                     /dnas_title="KZ101_P2A_CFP_polyA"
ORIGIN      
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataatAA
      661 AGTTGGTGGC GGATCcGGAG CTACTAATTT CTCTCTGTTA AAGCAGGCAG GAGATGTGGA
      721 GGAGAATCCA GGTCCTAAGG GAatggtgag caagggcgag gagctgttca ccggggtggt
      781 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      841 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      901 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag
      961 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1021 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1081 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1141 ggacggcaac atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat
     1201 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
     1261 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1321 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     1381 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     1441 catggacgag ctgtacaagt aagaattctg aagcttagcc tagagctcgc tgatcagcct
     1501 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     1561 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     1621 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     1681 attgggaaga gaatagcagg catgctgggg aggtacctga CCCAGCTTTc ttgtacaaag
     1741 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     1801 aaaataaaat cattatttgc catccagctg cagggcggcc gcgatatccc ctatagtgag
     1861 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     1921 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     1981 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     2041 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     2101 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     2161 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     2221 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     2281 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     2341 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     2401 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     2461 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     2521 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     2581 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     2641 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     2701 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     2761 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     2821 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     2881 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     2941 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3001 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3061 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     3121 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3181 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3241 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3301 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3361 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3421 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3481 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3541 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3601 cacatgtt
//

