LOCUS pDONR221-P3P2 4774 bp DNA circular SYN 23-APR-2009 SOURCE ORGANISM COMMENT VNTDATE|391339286| COMMENT VNTDBDATE|416244976| COMMENT VNTNAME|pDONR221-P3P2| COMMENT VNTAUTHORNAME|Invitrogen| COMMENT VNTAUTHORTEL|(800)-357-3114| COMMENT VNTAUTHORFAX|(760)-602-6500| COMMENT VNTOAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com| COMMENT VNTAUTHOREML|bioinfosales@invitrogen.com| COMMENT VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics| COMMENT VNTAUTHORAD1|Invitrogen Corporation| COMMENT VNTAUTHORAD2|1620 Faraday Avenue| COMMENT VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.| FEATURES Location/Qualifiers misc_recomb 570..667 /label=attL3 /note="attL3 recombination site" /note="recognized as attL3" misc_recomb complement(2136..2233) /label=attL2 /note="attL2 recombination site" /note="recognized as attL2" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence" misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence" primer_bind 537..552 /note="M13 Forward (-20) primer binding site" primer_bind 517..533 /note="M13 Forward (-40) primer binding site" primer_bind complement(2288..2304) /note="M13 Reverse primer binding site" rep_origin 3347..4020 /note="f1 origin" gene 2417..3226 /note="Kanamycin resistance gene" vector join(2145..4023,1..658) /source="pDONR221-P3P2" /type="Donor Vector" misc_feature 680..736 /note="P2A self-cleaving peptide" misc_feature 731..736 /note="G^P cleavage site" misc_feature 671..679 /note="GSG linker" gene 743..1459 /note="eGFP" terminator 1514..2111 /note="WPRE" source 1..4023 /dnas_title="XX001_P2A_eGFP_WPRE" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataatAA 661 AGTTGGTGGC gGATCcGGAG CTACTAATTT CTCTCTGTTA AAGCAGGCAG GAGATGTGGA 721 GGAGAATCCA GGTCCTAAGG gaATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT 781 GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA 841 GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA 901 GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG 961 CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA 1021 CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT 1081 GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA 1141 GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT 1201 CATGGCCGAC AAGCAGAAGA ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA 1261 GGACGGCAGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC 1321 CGTGCTGCTG CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA 1381 CGAGAAGCGC GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG 1441 CATGGACGAG CTGTACAAGt aaGaattctg aagcttagcc tagagctcct atagtgagAC 1501 CGGTTAGTAA TGATCGACAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT 1561 ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT 1621 CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG 1681 TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT 1741 GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT 1801 TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC 1861 TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG 1921 TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC 1981 TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG 2041 CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC 2101 TCCCCGCCTG GCGATGGTAC tgtcctagta cctgaCCCAG CTTTcttgta caaagttggc 2161 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat 2221 aaaatcatta tttgccatcc agctgcaggg cggccgcgat atcccctata gtgagtcgta 2281 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta 2341 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag 2401 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc 2461 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg 2521 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg 2581 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga 2641 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact 2701 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg 2761 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg 2821 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa 2881 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca 2941 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg 3001 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt 3061 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg 3121 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga 3181 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa 3241 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc 3301 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat 3361 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 3421 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 3481 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt 3541 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 3601 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 3661 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 3721 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 3781 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 3841 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 3901 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 3961 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 4021 gtt //