LOCUS pDONR221-P3P2 4774 bp DNA circular SYN 23-APR-2009 SOURCE ORGANISM COMMENT VNTDATE|391339286| COMMENT VNTDBDATE|416244976| COMMENT VNTNAME|pDONR221-P3P2| COMMENT VNTAUTHORNAME|Invitrogen| COMMENT VNTAUTHORTEL|(800)-357-3114| COMMENT VNTAUTHORFAX|(760)-602-6500| COMMENT VNTOAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com| COMMENT VNTAUTHOREML|bioinfosales@invitrogen.com| COMMENT VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics| COMMENT VNTAUTHORAD1|Invitrogen Corporation| COMMENT VNTAUTHORAD2|1620 Faraday Avenue| COMMENT VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.| FEATURES Location/Qualifiers misc_recomb 570..667 /label=attL3 /note="attL3 recombination site" /note="recognized as attL3" misc_recomb complement(2073..2170) /label=attL2 /note="attL2 recombination site" /note="recognized as attL2" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence" misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence" primer_bind 537..552 /note="M13 Forward (-20) primer binding site" primer_bind 517..533 /note="M13 Forward (-40) primer binding site" primer_bind complement(2225..2241) /note="M13 Reverse primer binding site" rep_origin 3284..3957 /note="f1 origin" gene 2354..3163 /note="Kanamycin resistance gene" vector join(2082..3960,1..658) /source="pDONR221-P3P2" /type="Donor Vector" gene 680..1399 /note="eCFP" misc_feature 665..679 /note="GGGGS linker" terminator 1451..2048 /note="WPRE" source 1..3960 /dnas_title="LD101_CFP_WPRE" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataatAA 661 AGTTGGTGGC GGcGGAtcca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 721 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 781 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 841 gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcagccg 901 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt 961 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 1021 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 1081 cggcaacatc ctggggcaca agctggagta caactacatc agccacaacg tctatatcac 1141 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga 1201 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 1261 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga 1321 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat 1381 ggacgagctg tacaagtaaG aattctgaag cttagcctag agctcctata gtgagACCGG 1441 TTAGTAATGA TCGACAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT 1501 CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT 1561 GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT 1621 CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT 1681 GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC 1741 GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG 1801 ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA GCTGACGTCC 1861 TTTCCATGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC 1921 GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG 1981 CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC 2041 CCGCCTGGCG ATGGTACtgt cctagtacct gaCCCAGCTT Tcttgtacaa agttggcatt 2101 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa 2161 atcattattt gccatccagc tgcagggcgg ccgcgatatc ccctatagtg agtcgtatta 2221 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat 2281 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa 2341 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa 2401 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc 2461 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa 2521 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt 2581 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac 2641 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga 2701 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa 2761 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa 2821 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt 2881 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga 2941 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg 3001 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga 3061 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat 3121 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg 3181 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa 3241 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa 3301 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 3361 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 3421 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg 3481 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 3541 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 3601 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 3661 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 3721 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 3781 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 3841 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 3901 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt //