LOCUS pDONR221-P3P2 4774 bp DNA circular SYN 23-APR-2009 SOURCE ORGANISM COMMENT VNTDATE|391339286| COMMENT VNTDBDATE|416244976| COMMENT VNTNAME|pDONR221-P3P2| COMMENT VNTAUTHORNAME|Invitrogen| COMMENT VNTAUTHORTEL|(800)-357-3114| COMMENT VNTAUTHORFAX|(760)-602-6500| COMMENT VNTOAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com| COMMENT VNTAUTHOREML|bioinfosales@invitrogen.com| COMMENT VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics| COMMENT VNTAUTHORAD1|Invitrogen Corporation| COMMENT VNTAUTHORAD2|1620 Faraday Avenue| COMMENT VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.| FEATURES Location/Qualifiers misc_recomb 570..667 /label=attL3 /note="attL3 recombination site" /note="recognized as attL3" misc_recomb complement(1601..1698) /label=attL2 /note="attL2 recombination site" /note="recognized as attL2" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence" misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence" primer_bind 537..552 /note="M13 Forward (-20) primer binding site" primer_bind 517..533 /note="M13 Forward (-40) primer binding site" primer_bind complement(1753..1769) /note="M13 Reverse primer binding site" rep_origin 2812..3485 /note="f1 origin" gene 1882..2691 /note="Kanamycin resistance gene" polyA_signal 1381..1591 /note="bGH Poly A " /note="bovine growth hormone polyadenylation signal" vector join(1610..3488,1..658) /source="pDONR221-P3P2" /type="Donor Vector" misc_feature 671..679 /note="GSG linker" misc_feature 680..736 /note="P2A self-cleaving peptide" misc_feature 731..736 /note="G^P cleavage site" gene 743..1342 /note="Puromycin resistance gene" source 1..3488 /dnas_title="KZ501_P2A_Puro_polyA" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataatAA 661 AGTTGGTGGC GGATCcGGAG CTACTAATTT CTCTCTGTTA AAGCAGGCAG GAGATGTGGA 721 GGAGAATCCA GGTCCTAAGG GAatgaccga gtacaagcct accgtgcgcc tggccactcg 781 cgatgatgtg ccccgcgccg tccgcactct ggccgccgct ttcgccgact accccgctac 841 ccggcacacc gtggaccccg accggcacat cgagcgtgtg acagagttgc aggagctgtt 901 cctgacccgc gtcgggctgg acatcggcaa ggtgtgggta gccgacgacg gcgcggccgt 961 ggccgtgtgg actacccccg agagcgttga ggccggcgcc gtgttcgccg agatcggccc 1021 ccgaatggcc gagctgagcg gcagccgcct ggccgcccag cagcaaatgg agggcctgct 1081 tgccccccat cgtcccaagg agcctgcctg gtttctggcc actgtaggag tgagccccga 1141 ccaccagggc aagggcttgg gcagcgccgt cgtgttgccc ggcgtagagg ccgccgaacg 1201 cgccggtgtg cccgcctttc tcgaaacaag cgcaccaaga aaccttccat tctacgagcg 1261 cctgggcttc accgtgaccg ccgatgtcga ggtgcccgag ggacctagga cctggtgtat 1321 gacacgaaaa cctggcgcct aagaattctg aagcttagcc tagagctcgc tgatcagcct 1381 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga 1441 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt 1501 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg 1561 attgggaaga gaatagcagg catgctgggg aggtacctga CCCAGCTTTc ttgtacaaag 1621 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc 1681 aaaataaaat cattatttgc catccagctg cagggcggcc gcgatatccc ctatagtgag 1741 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga 1801 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata 1861 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta 1921 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa 1981 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa 2041 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg 2101 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg 2161 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat 2221 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct 2281 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga 2341 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt 2401 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact 2461 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt 2521 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc 2581 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat 2641 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg 2701 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca 2761 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa 2821 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 2881 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 2941 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 3001 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 3061 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 3121 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 3181 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 3241 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 3301 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 3361 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 3421 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 3481 cacatgtt //