LOCUS pDONR221-P3P2 4774 bp DNA circular SYN 23-APR-2009 SOURCE ORGANISM COMMENT VNTDATE|391339286| COMMENT VNTDBDATE|416244976| COMMENT VNTNAME|pDONR221-P3P2| COMMENT VNTAUTHORNAME|Invitrogen| COMMENT VNTAUTHORTEL|(800)-357-3114| COMMENT VNTAUTHORFAX|(760)-602-6500| COMMENT VNTOAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com| COMMENT VNTAUTHOREML|bioinfosales@invitrogen.com| COMMENT VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics| COMMENT VNTAUTHORAD1|Invitrogen Corporation| COMMENT VNTAUTHORAD2|1620 Faraday Avenue| COMMENT VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.| FEATURES Location/Qualifiers misc_recomb 570..667 /label=attL3 /note="attL3 recombination site" /note="recognized as attL3" misc_recomb complement(2039..2136) /label=attL2 /note="attL2 recombination site" /note="recognized as attL2" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence" misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence" primer_bind 537..552 /note="M13 Forward (-20) primer binding site" primer_bind 517..533 /note="M13 Forward (-40) primer binding site" primer_bind complement(2191..2207) /note="M13 Reverse primer binding site" rep_origin 3250..3923 /note="f1 origin" gene 2320..3129 /note="Kanamycin resistance gene" polyA_signal 1819..2029 /note="bGH Poly A " /note="bovine growth hormone polyadenylation signal" vector join(2048..3926,1..658) /source="pDONR221-P3P2" /type="Donor Vector" misc_feature 671..679 /note="GSG linker" misc_feature 680..736 /note="P2A self cleavage peptide" misc_feature 731..736 /note="G^P cleavage site" gene 743..1780 /gene="Hygromycin resistance gene" source 1..3926 /dnas_title="KZ201_P2A_Hygro_polyA" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataatAA 661 AGTTGGTGGC GGATCcGGAG CTACTAATTT CTCTCTGTTA AAGCAGGCAG GAGATGTGGA 721 GGAGAATCCA GGTCCTAAGG GAatgaagaa gcccgaactc accgctacca gcgttgaaaa 781 atttctcatc gagaagttcg acagtgtgag cgacctgatg cagttgtcgg agggcgaaga 841 gagccgagcc ttcagcttcg atgtcggcgg acgcggctat gtactgcggg tgaatagctg 901 cgctgatggc ttctacaaag accgctacgt gtaccgccac ttcgccagcg ctgcactacc 961 catccccgaa gtgttggaca tcggcgagtt cagcgagagc ctgacatact gcatcagtag 1021 acgcgcccaa ggcgttactc tccaagacct ccccgaaaca gagctgcctg ctgtgttaca 1081 gcctgtcgcc gaagctatgg atgctattgc cgccgccgac ctcagtcaaa ccagcggctt 1141 cggcccattc gggccccaag gcatcggcca gtacacaacc tggcgggatt tcatttgcgc 1201 cattgctgat ccccatgtct accactggca gaccgtgatg gacgacaccg tgtccgccag 1261 cgtagctcaa gccctggacg aactgatgct gtgggccgaa gactgtcccg aggtgcgcca 1321 cctcgtccat gccgacttcg gcagcaacaa cgtcctgacc gacaacggcc gcatcaccgc 1381 cgtaatcgac tggtccgaag ctatgttcgg ggacagtcag tacgaggtgg ccaacatctt 1441 cttctggcgg ccctggctgg cttgcatgga gcagcagact cgctacttcg agcgccggca 1501 tcccgagctg gccggcagcc ctcgtctgcg agcctacatg ctgcgcatcg gcctggatca 1561 gctctaccag agcctcgtgg acggcaactt cgacgatgct gcctgggctc aaggccgctg 1621 cgatgccatc gtccgcagcg gggccggcac cgtcggtcgc acacaaatcg ctcgccggag 1681 cgcagccgta tggaccgacg gctgcgtcga ggtgctggcc gacagcggca accgccggcc 1741 cagtacacga ccgcgcgcta aggaggtagg tcgagtttaa gaattctgaa gcttagccta 1801 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 1861 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 1921 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 1981 aggacagcaa gggggaggat tgggaagaga atagcaggca tgctggggag gtacctgaCC 2041 CAGCTTTctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg 2101 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgca gggcggccgc 2161 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc 2221 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata 2281 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa 2341 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct 2401 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg 2461 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg 2521 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt 2581 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta 2641 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc 2701 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc 2761 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag 2821 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca 2881 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg 2941 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt 3001 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa 3061 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag 3121 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga 3181 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga 3241 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 3301 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 3361 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 3421 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 3481 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 3541 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 3601 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 3661 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 3721 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 3781 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 3841 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 3901 ttttgctggc cttttgctca catgtt //