LOCUS       pDONR221-P3P2           4774 bp    DNA     circular SYN 23-APR-2009
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|391339286|
COMMENT     VNTDBDATE|416244976|
COMMENT     VNTNAME|pDONR221-P3P2|
COMMENT     VNTAUTHORNAME|Invitrogen|
COMMENT     VNTAUTHORTEL|(800)-357-3114|
COMMENT     VNTAUTHORFAX|(760)-602-6500|
COMMENT     VNTOAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com|
COMMENT     VNTAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics|
COMMENT     VNTAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
FEATURES             Location/Qualifiers
     misc_recomb     570..667
                     /label=attL3
                     /note="attL3 recombination site"
                     /note="recognized as attL3"
     misc_recomb     complement(1376..1473)
                     /label=attL2
                     /note="attL2 recombination site"
                     /note="recognized as attL2"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence"
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence"
     primer_bind     537..552
                     /note="M13 Forward (-20) primer binding site"
     primer_bind     517..533
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     complement(1528..1544)
                     /note="M13 Reverse primer binding site"
     rep_origin      2587..3260
                     /note="f1 origin"
     gene            1657..2466
                     /note="Kanamycin resistance gene"
     polyA_signal    1156..1366
                     /note="bGH Poly A "
                     /note="bovine growth hormone polyadenylation signal"
     vector          join(1385..3263,1..658)
                     /source="pDONR221-P3P2"
                     /type="Donor Vector"
     misc_feature    671..679
                     /note="GSG linker"
     misc_feature    680..736
                     /note="P2A self-cleaving peptide"
     misc_feature    731..736
                     /note="G^P cleavage site"
     gene            743..1117
                     /note="Zeocin resistance gene"
     source          1..3263
                     /dnas_title="KZ601_P2A_Zeo_polyA"
ORIGIN      
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataatAA
      661 AGTTGGTGGC GGATCcGGAG CTACTAATTT CTCTCTGTTA AAGCAGGCAG GAGATGTGGA
      721 GGAGAATCCA GGTCCTAAGG GAatggccaa gttgaccagt gccgttccgg tgctcaccgc
      781 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
      841 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
      901 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg ttggtgcgcg gcctggacga
      961 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     1021 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     1081 caactgcgtg cacttcgtgg ccgaggagca ggactaagaa ttctgaagct tagcctagag
     1141 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     1201 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     1261 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     1321 acagcaaggg ggaggattgg gaagagaata gcaggcatgc tggggaggta cctgaCCCAG
     1381 CTTTcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     1441 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgcaggg cggccgcgat
     1501 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     1561 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     1621 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     1681 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     1741 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     1801 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     1861 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     1921 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     1981 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     2041 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     2101 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     2161 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     2221 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     2281 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     2341 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     2401 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     2461 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     2521 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     2581 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2641 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2701 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2761 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2821 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2881 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2941 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3001 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3061 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3121 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3181 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3241 tgctggcctt ttgctcacat gtt
//

