LOCUS pDONR221-P3P2 4774 bp DNA circular SYN 23-APR-2009 SOURCE ORGANISM COMMENT VNTDATE|391339286| COMMENT VNTDBDATE|416244976| COMMENT VNTNAME|pDONR221-P3P2| COMMENT VNTAUTHORNAME|Invitrogen| COMMENT VNTAUTHORTEL|(800)-357-3114| COMMENT VNTAUTHORFAX|(760)-602-6500| COMMENT VNTOAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com| COMMENT VNTAUTHOREML|bioinfosales@invitrogen.com| COMMENT VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics| COMMENT VNTAUTHORAD1|Invitrogen Corporation| COMMENT VNTAUTHORAD2|1620 Faraday Avenue| COMMENT VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.| FEATURES Location/Qualifiers misc_recomb 570..667 /label=attL3 /note="attL3 recombination site" /note="recognized as attL3" misc_recomb complement(2016..2113) /label=attL2 /note="attL2 recombination site" /note="recognized as attL2" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence" misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence" primer_bind 537..552 /note="M13 Forward (-20) primer binding site" primer_bind 517..533 /note="M13 Forward (-40) primer binding site" primer_bind complement(2168..2184) /note="M13 Reverse primer binding site" rep_origin 3227..3900 /note="f1 origin" gene 2297..3106 /note="Kanamycin resistance gene" vector join(2025..3903,1..658) /source="pDONR221-P3P2" /type="Donor Vector" misc_feature 680..736 /note="P2A self-cleaving peptide" misc_feature 731..736 /note="G^P cleavage site" misc_feature 671..679 /note="GSG linker" gene 743..1342 /note="Puromycin resistance gene" terminator 1394..1991 /note="WPRE" source 1..3903 /dnas_title="LA601_P2A_Puro_WPRE" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataatAA 661 AGTTGGTGGC gGATCcGGAG CTACTAATTT CTCTCTGTTA AAGCAGGCAG GAGATGTGGA 721 GGAGAATCCA GGTCCTAAGG gaatgaccga gtacaagcct accgtgcgcc tggccactcg 781 cgatgatgtg ccccgcgccg tccgcactct ggccgccgct ttcgccgact accccgctac 841 ccggcacacc gtggaccccg accggcacat cgagcgtgtg acagagttgc aggagctgtt 901 cctgacccgc gtcgggctgg acatcggcaa ggtgtgggta gccgacgacg gcgcggccgt 961 ggccgtgtgg actacccccg agagcgttga ggccggcgcc gtgttcgccg agatcggccc 1021 ccgaatggcc gagctgagcg gcagccgcct ggccgcccag cagcaaatgg agggcctgct 1081 tgccccccat cgtcccaagg agcctgcctg gtttctggcc actgtaggag tgagccccga 1141 ccaccagggc aagggcttgg gcagcgccgt cgtgttgccc ggcgtagagg ccgccgaacg 1201 cgccggtgtg cccgcctttc tcgaaacaag cgcaccaaga aaccttccat tctacgagcg 1261 cctgggcttc accgtgaccg ccgatgtcga ggtgcccgag ggacctagga cctggtgtat 1321 gacacgaaaa cctggcgcct aaGaattctg aagcttagcc tagagctcct atagtgagAC 1381 CGGTTAGTAA TGATCGACAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT 1441 ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT 1501 CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG 1561 TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT 1621 GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT 1681 TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC 1741 TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG 1801 TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC 1861 TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG 1921 CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC 1981 TCCCCGCCTG GCGATGGTAC tgtcctagta cctgaCCCAG CTTTcttgta caaagttggc 2041 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat 2101 aaaatcatta tttgccatcc agctgcaggg cggccgcgat atcccctata gtgagtcgta 2161 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta 2221 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag 2281 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc 2341 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg 2401 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg 2461 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga 2521 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact 2581 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg 2641 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg 2701 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa 2761 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca 2821 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg 2881 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt 2941 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg 3001 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga 3061 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa 3121 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc 3181 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat 3241 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 3301 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 3361 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt 3421 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 3481 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 3541 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 3601 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 3661 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 3721 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 3781 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 3841 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 3901 gtt //