LOCUS       pDONR221-P3P2           4774 bp    DNA     circular SYN 23-APR-2009
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|391339286|
COMMENT     VNTDBDATE|416244976|
COMMENT     VNTNAME|pDONR221-P3P2|
COMMENT     VNTAUTHORNAME|Invitrogen|
COMMENT     VNTAUTHORTEL|(800)-357-3114|
COMMENT     VNTAUTHORFAX|(760)-602-6500|
COMMENT     VNTOAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com|
COMMENT     VNTAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics|
COMMENT     VNTAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
FEATURES             Location/Qualifiers
     misc_recomb     570..667
                     /label=attL3
                     /note="attL3 recombination site"
                     /note="recognized as attL3"
     misc_recomb     complement(2016..2113)
                     /label=attL2
                     /note="attL2 recombination site"
                     /note="recognized as attL2"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence"
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence"
     primer_bind     537..552
                     /note="M13 Forward (-20) primer binding site"
     primer_bind     517..533
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     complement(2168..2184)
                     /note="M13 Reverse primer binding site"
     rep_origin      3227..3900
                     /note="f1 origin"
     gene            2297..3106
                     /note="Kanamycin resistance gene"
     vector          join(2025..3903,1..658)
                     /source="pDONR221-P3P2"
                     /type="Donor Vector"
     misc_feature    680..736
                     /note="P2A self-cleaving peptide"
     misc_feature    731..736
                     /note="G^P cleavage site"
     misc_feature    671..679
                     /note="GSG linker"
     gene            743..1342
                     /note="Puromycin resistance gene"
     terminator      1394..1991
                     /note="WPRE"
     source          1..3903
                     /dnas_title="LA601_P2A_Puro_WPRE"
ORIGIN      
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataatAA
      661 AGTTGGTGGC gGATCcGGAG CTACTAATTT CTCTCTGTTA AAGCAGGCAG GAGATGTGGA
      721 GGAGAATCCA GGTCCTAAGG gaatgaccga gtacaagcct accgtgcgcc tggccactcg
      781 cgatgatgtg ccccgcgccg tccgcactct ggccgccgct ttcgccgact accccgctac
      841 ccggcacacc gtggaccccg accggcacat cgagcgtgtg acagagttgc aggagctgtt
      901 cctgacccgc gtcgggctgg acatcggcaa ggtgtgggta gccgacgacg gcgcggccgt
      961 ggccgtgtgg actacccccg agagcgttga ggccggcgcc gtgttcgccg agatcggccc
     1021 ccgaatggcc gagctgagcg gcagccgcct ggccgcccag cagcaaatgg agggcctgct
     1081 tgccccccat cgtcccaagg agcctgcctg gtttctggcc actgtaggag tgagccccga
     1141 ccaccagggc aagggcttgg gcagcgccgt cgtgttgccc ggcgtagagg ccgccgaacg
     1201 cgccggtgtg cccgcctttc tcgaaacaag cgcaccaaga aaccttccat tctacgagcg
     1261 cctgggcttc accgtgaccg ccgatgtcga ggtgcccgag ggacctagga cctggtgtat
     1321 gacacgaaaa cctggcgcct aaGaattctg aagcttagcc tagagctcct atagtgagAC
     1381 CGGTTAGTAA TGATCGACAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT
     1441 ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT
     1501 CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG
     1561 TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT
     1621 GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT
     1681 TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC
     1741 TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG
     1801 TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC
     1861 TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG
     1921 CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC
     1981 TCCCCGCCTG GCGATGGTAC tgtcctagta cctgaCCCAG CTTTcttgta caaagttggc
     2041 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     2101 aaaatcatta tttgccatcc agctgcaggg cggccgcgat atcccctata gtgagtcgta
     2161 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     2221 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     2281 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     2341 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     2401 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     2461 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     2521 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     2581 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     2641 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     2701 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     2761 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     2821 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     2881 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     2941 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     3001 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     3061 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     3121 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     3181 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     3241 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3301 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3361 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3421 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3481 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3541 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3601 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3661 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3721 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3781 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3841 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3901 gtt
//

