LOCUS       Exported                3137 bp ds-DNA     circular SYN 17-JUL-2006
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDONR221-P3P2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Invitrogen
  TITLE     Direct Submission
  JOURNAL   Exported Jan 17, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=KK501_FKBP DD_bGHpolyA"
     misc_feature    complement(268..295)
                     /label=rrnB T2 transcription termination sequence
                     /note="rrnB T2 transcription termination sequence"
     misc_feature    complement(427..470)
                     /label=rrnB T1 transcription termination sequence
                     /note="rrnB T1 transcription termination sequence"
     primer_bind     517..533
                     /label=M13 Forward (-40) primer binding site
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     537..552
                     /label=M13 Forward (-20) primer binding site
                     /note="M13 Forward (-20) primer binding site"
     misc_recomb     570..667
                     /label=attL3
                     /note="attL3 recombination site"
                     /note="recognized as attL3"
     misc_feature    665..679
                     /label=GGGGS linker
                     /note="GGGGS linker"
     gene            680..1000
                     /label=FKBP destabilization domain
                     /note="FKBP destabilization domain"
     polyA_signal    1030..1240
                     /label=bGH Poly A
                     /note="bGH Poly A"
                     /note="bovine growth hormone polyadenylation signal"
     misc_recomb     complement(1250..1347)
                     /label=attL2
                     /note="attL2 recombination site"
                     /note="recognized as attL2"
     misc_feature    join(1259..3137,1..658)
                     /label=nonstandard type: vector
                     /note="/source=pDONR221-P3P2"
                     /note="/type=Donor Vector"
     primer_bind     complement(1402..1418)
                     /label=M13 Reverse primer binding site
                     /note="M13 Reverse primer binding site"
     gene            1531..2340
                     /label=Kanamycin resistance gene
                     /note="Kanamycin resistance gene"
     rep_origin      2461..3134
                     /label=f1 origin
                     /note="f1 origin"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataataa
      661 agttggtggc ggaggatccg gagtgcaggt ggaaaccatc tccccaggag acgggcgcac
      721 cttccccaag cgcggccaga cctgcgtggt gcactacacc gggatgcttg gagatggaaa
      781 gaaagttgac tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga
      841 ggtgatccga ggctgggaag aaggggttgc ccagatgagt gtgggtcagg gagccaaact
      901 gactatatct ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca
      961 tgccactctc gtcttcgatg tggagcttct agaactggaa tagaattcct agagctcgct
     1021 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     1081 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     1141 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     1201 agggggagga ttgggaagag aatagcaggc atgctgggga ggtacctgac ccagctttct
     1261 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     1321 ctatcagtca aaataaaatc attatttgcc atccagctgc agggcggccg cgatatcccc
     1381 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     1441 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
     1501 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
     1561 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     1621 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
     1681 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     1741 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     1801 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
     1861 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     1921 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
     1981 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     2041 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     2101 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     2161 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     2221 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     2281 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     2341 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     2401 gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     2461 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2521 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2581 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2641 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2701 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2761 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2821 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2881 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2941 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3001 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3061 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3121 ccttttgctc acatgtt
//
