LOCUS       Exported                3290 bp ds-DNA     circular SYN 17-JUL-2006
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDONR221-P3P2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Invitrogen
  TITLE     Direct Submission
  JOURNAL   Exported Jan 17, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=KK601_ecDHFR DD_bGHpolyA"
     misc_feature    complement(268..295)
                     /label=rrnB T2 transcription termination sequence
                     /note="rrnB T2 transcription termination sequence"
     misc_feature    complement(427..470)
                     /label=rrnB T1 transcription termination sequence
                     /note="rrnB T1 transcription termination sequence"
     primer_bind     517..533
                     /label=M13 Forward (-40) primer binding site
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     537..552
                     /label=M13 Forward (-20) primer binding site
                     /note="M13 Forward (-20) primer binding site"
     misc_recomb     570..667
                     /label=attL3
                     /note="attL3 recombination site"
                     /note="recognized as attL3"
     misc_feature    665..679
                     /label=GGGGS linker
                     /note="GGGGS linker"
     gene            680..1156
                     /label=ecDHFR destabilization domain
                     /note="ecDHFR destabilization domain"
     polyA_signal    1183..1393
                     /label=bGH Poly A
                     /note="bGH Poly A"
                     /note="bovine growth hormone polyadenylation signal"
     misc_recomb     complement(1403..1500)
                     /label=attL2
                     /note="attL2 recombination site"
                     /note="recognized as attL2"
     misc_feature    join(1412..3290,1..658)
                     /label=nonstandard type: vector
                     /note="/source=pDONR221-P3P2"
                     /note="/type=Donor Vector"
     primer_bind     complement(1555..1571)
                     /label=M13 Reverse primer binding site
                     /note="M13 Reverse primer binding site"
     gene            1684..2493
                     /label=Kanamycin resistance gene
                     /note="Kanamycin resistance gene"
     rep_origin      2614..3287
                     /label=f1 origin
                     /note="f1 origin"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataataa
      661 agttggtggc ggaggatcca tcagtctgat tgcggcgtta gcggtagatc acgttatcgg
      721 catggaaacc gtcatgccgt ggaacctgcc tgccgatctc gcctggttta aacgcaacac
      781 cttaaataaa cccgtgatta tgggccgcca tacctgggaa tcaatcggtc gtccgttgcc
      841 aggacgcaaa aatattatcc tcagcagtca accgagtacg gacgatcgcg taacgtgggt
      901 gaagtcggtg gatgaagcca tcgcggcgtg tggtgacgta ccagaaatca tggttattgg
      961 cggcggtcgc gtttatgaac agttcttgcc aaaagcgcaa aaactgtatc tgacgcatat
     1021 cgacgcagaa gtggaaggcg acacccattt cccggattac gagccggatg actgggaatc
     1081 ggtattcagc gaattccacg atgctgatgc gcagaactct cacagctatt gctttgagat
     1141 tctggagcgg cgatgaaatt cctagagctc gctgatcagc ctcgactgtg ccttctagtt
     1201 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     1261 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     1321 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gagaatagca
     1381 ggcatgctgg ggaggtacct gacccagctt tcttgtacaa agttggcatt ataagaaagc
     1441 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     1501 gccatccagc tgcagggcgg ccgcgatatc ccctatagtg agtcgtatta catggtcata
     1561 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     1621 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     1681 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     1741 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     1801 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     1861 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     1921 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     1981 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     2041 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     2101 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     2161 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     2221 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     2281 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     2341 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     2401 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     2461 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     2521 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     2581 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2641 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2701 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2761 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2821 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2881 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2941 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3001 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3061 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3121 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3181 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3241 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
