LOCUS       pDONR221-P3P2           4774 bp    DNA     circular SYN 23-APR-2009
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|391339286|
COMMENT     VNTDBDATE|416244976|
COMMENT     VNTNAME|pDONR221-P3P2|
COMMENT     VNTAUTHORNAME|Invitrogen|
COMMENT     VNTAUTHORTEL|(800)-357-3114|
COMMENT     VNTAUTHORFAX|(760)-602-6500|
COMMENT     VNTOAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com|
COMMENT     VNTAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics|
COMMENT     VNTAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
FEATURES             Location/Qualifiers
     misc_recomb     570..667
                     /label=attL3
                     /note="attL3 recombination site"
                     /note="recognized as attL3"
     misc_recomb     complement(1145..1242)
                     /label=attL2
                     /note="attL2 recombination site"
                     /note="recognized as attL2"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence"
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence"
     primer_bind     537..552
                     /note="M13 Forward (-20) primer binding site"
     primer_bind     517..533
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     complement(1297..1313)
                     /note="M13 Reverse primer binding site"
     rep_origin      2356..3029
                     /note="f1 origin"
     gene            1426..2235
                     /note="Kanamycin resistance gene"
     polyA_signal    925..1135
                     /note="bGH Poly A "
                     /note="bovine growth hormone polyadenylation signal"
     vector          join(1154..3032,1..658)
                     /source="pDONR221-P3P2"
                     /type="Donor Vector"
     gene            680..883
                     /note="mAID "
                     /note="derived from PMID: 27052166"
     misc_feature    665..679
                     /note="GGGGS linker"
     source          1..3032
                     /dnas_title="XX001_mAID_polyA"
ORIGIN      
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataatAA
      661 AGTTGGTGGC GGcGGAtccA AGGAGAAGAG TGCTTGTCCT AAAGATCCAG CCAAACCTCC
      721 GGCCAAGGCA CAAGTTGTGG GATGGCCACC GGTGAGATCA TACCGGAAGA ACGTGATGGT
      781 TTCCTGCCAA AAATCAAGCG GTGGCCCGGA GGCGGCGGCG TTCGTGAAGG TATCAATGGA
      841 CGGAGCACCG TACTTGAGGA AAATCGATTT GAGGATGTAT AAAtaagaat tctgaagctt
      901 agcctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
      961 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     1021 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     1081 tggggcagga cagcaagggg gaggattggg aagagaatag caggcatgct ggggaggtac
     1141 ctgaCCCAGC TTTcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
     1201 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgcagggc
     1261 ggccgcgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     1321 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     1381 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     1441 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     1501 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     1561 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     1621 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     1681 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     1741 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     1801 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     1861 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     1921 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     1981 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     2041 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     2101 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     2161 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     2221 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     2281 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     2341 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2401 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2461 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2521 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2581 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2641 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2701 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2761 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2821 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2881 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2941 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3001 ctggcctttt gctggccttt tgctcacatg tt
//

