LOCUS       pDONR221-P3P2           4774 bp    DNA     circular SYN 23-APR-2009
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|391339286|
COMMENT     VNTDBDATE|416244976|
COMMENT     VNTNAME|pDONR221-P3P2|
COMMENT     VNTAUTHORNAME|Invitrogen|
COMMENT     VNTAUTHORTEL|(800)-357-3114|
COMMENT     VNTAUTHORFAX|(760)-602-6500|
COMMENT     VNTOAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com|
COMMENT     VNTAUTHOREML|bioinfosales@invitrogen.com|
COMMENT     VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics|
COMMENT     VNTAUTHORAD1|Invitrogen Corporation|
COMMENT     VNTAUTHORAD2|1620 Faraday Avenue|
COMMENT     VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
FEATURES             Location/Qualifiers
     misc_recomb     570..667
                     /label=attL3
                     /note="attL3 recombination site"
                     /note="recognized as attL3"
     misc_recomb     complement(1560..1657)
                     /label=attL2
                     /note="attL2 recombination site"
                     /note="recognized as attL2"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence"
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence"
     primer_bind     537..552
                     /note="M13 Forward (-20) primer binding site"
     primer_bind     517..533
                     /note="M13 Forward (-40) primer binding site"
     primer_bind     complement(1712..1728)
                     /note="M13 Reverse primer binding site"
     rep_origin      2771..3444
                     /note="f1 origin"
     gene            1841..2650
                     /note="Kanamycin resistance gene"
     vector          join(1569..3447,1..658)
                     /source="pDONR221-P3P2"
                     /type="Donor Vector"
     gene            680..883
                     /note="mAID "
                     /note="derived from PMID: 27052166"
     terminator      938..1535
                     /note="WPRE"
     gene            665..679
                     /note="GGGGS linker"
     source          1..3447
                     /dnas_title="LA801_mAID_WPRE"
ORIGIN      
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataatAA
      661 AGTTGGTGGC GGcGGAtccA AGGAGAAGAG TGCTTGTCCT AAAGATCCAG CCAAACCTCC
      721 GGCCAAGGCA CAAGTTGTGG GATGGCCACC GGTGAGATCA TACCGGAAGA ACGTGATGGT
      781 TTCCTGCCAA AAATCAAGCG GTGGCCCGGA GGCGGCGGCG TTCGTGAAGG TATCAATGGA
      841 CGGAGCACCG TACTTGAGGA AAATCGATTT GAGGATGTAT AAAtaaGaat tctgaagctt
      901 agcctagagc tcctatagtg agACCGGTTA GTAATGATCG ACAATCAACC TCTGGATTAC
      961 AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA
     1021 TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC
     1081 TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA
     1141 CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC
     1201 ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC
     1261 ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC
     1321 GTGGTGTTGT CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG
     1381 ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT
     1441 TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG
     1501 AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CCTGGCGATG GTACtgtcct agtacctgaC
     1561 CCAGCTTTct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
     1621 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctgc agggcggccg
     1681 cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     1741 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     1801 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     1861 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     1921 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     1981 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     2041 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     2101 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     2161 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     2221 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     2281 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     2341 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     2401 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     2461 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     2521 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     2581 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     2641 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     2701 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     2761 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2821 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2881 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2941 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3001 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3061 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3121 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3181 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3241 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3301 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3361 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3421 cttttgctgg ccttttgctc acatgtt
//

