LOCUS Exported 3573 bp ds-DNA circular SYN 17-JUL-2006 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pDONR221-P3P2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3573) AUTHORS Invitrogen TITLE Direct Submission JOURNAL Exported Jan 17, 2019 from SnapGene Viewer 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3573 /organism="synthetic DNA construct" /note="/dnas_title=IY202_APEX2_bGHpolyA" misc_feature complement(268..295) /label=rrnB T2 transcription termination sequence /note="rrnB T2 transcription termination sequence" misc_feature complement(427..470) /label=rrnB T1 transcription termination sequence /note="rrnB T1 transcription termination sequence" primer_bind 517..533 /label=M13 Forward (-40) primer binding site /note="M13 Forward (-40) primer binding site" primer_bind 537..552 /label=M13 Forward (-20) primer binding site /note="M13 Forward (-20) primer binding site" misc_recomb 570..667 /label=attL3 /note="attL3 recombination site" /note="recognized as attL3" misc_feature 665..691 /label=flexible linker /note="flexible linker" gene 692..1435 /label=APEX2 /note="APEX2" /note="derived from Addgene #49386" polyA_signal 1466..1676 /label=bGH Poly A /note="bGH Poly A" /note="shorter sequence than in pEF-DEST51 (invitrogen)" misc_recomb complement(1686..1783) /label=attL2 /note="attL2 recombination site" /note="recognized as attL2" misc_feature join(1695..3573,1..658) /label=nonstandard type: vector /note="/source=pDONR221-P3P2" /note="/type=Donor Vector" primer_bind complement(1838..1854) /label=M13 Reverse primer binding site /note="M13 Reverse primer binding site" gene 1967..2776 /label=Kanamycin resistance gene /note="Kanamycin resistance gene" rep_origin 2897..3570 /label=f1 origin /note="f1 origin" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataataa 661 agttggtggc ggatccggcg gaggtggatc taagtcttac ccaactgtga gtgctgatta 721 ccaggacgcc gttgagaagg cgaagaagaa gctcagaggc ttcatcgctg agaagagatg 781 cgctcctcta atgctccgtt tggcattcca ctctgctgga acctttgaca agggcacgaa 841 gaccggtgga cccttcggaa ccatcaagca ccctgccgaa ctggctcaca gcgctaacaa 901 cggtcttgac atcgctgtta ggcttttgga gccactcaag gcggagttcc ctattttgag 961 ctacgccgat ttctaccagt tggctggcgt tgttgccgtt gaggtcacgg gtggacctaa 1021 ggttccattc caccctggaa gagaggacaa gcctgagccg ccaccagagg gtcgcttgcc 1081 cgatcccact aagggttctg accatttgag agatgtgttt ggcaaagcta tggggcttac 1141 tgaccaagat atcgttgctc tatctggggg tcacactatt ggagctgcac acaaggagcg 1201 ttctggattt gagggtccct ggacctctaa tcctcttatt ttcgacaact catacttcac 1261 ggagttgttg agtggtgaga aggaaggtct ccttcagcta ccttctgaca aggctctttt 1321 gtctgaccct gtattccgcc ctctcgttga caaatatgca gcggacgaag atgccttctt 1381 tgctgattac gctgaggctc accaaaagct ttccgagctt gggtttgctg atgcctaaga 1441 attcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 1501 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 1561 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 1621 gtggggcagg acagcaaggg ggaggattgg gaagagaata gcaggcatgc tggggaggta 1681 cctgacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt 1741 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgcaggg 1801 cggccgcgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct 1861 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg 1921 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca 1981 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa 2041 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc 2101 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga 2161 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt 2221 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt 2281 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt 2341 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt 2401 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga 2461 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc 2521 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga 2581 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca 2641 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct 2701 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct 2761 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct 2821 gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt 2881 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 2941 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 3001 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 3061 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 3121 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 3181 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 3241 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 3301 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 3361 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 3421 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 3481 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 3541 cctggccttt tgctggcctt ttgctcacat gtt //