LOCUS Exported 3988 bp ds-DNA circular SYN 17-JUL-2006 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pDONR221-P3P2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3988) AUTHORS Invitrogen TITLE Direct Submission JOURNAL Exported Jan 17, 2019 from SnapGene Viewer 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3988 /organism="synthetic DNA construct" /note="/dnas_title=JD602_APEX2_WPRE" misc_feature complement(268..295) /label=rrnB T2 transcription termination sequence /note="rrnB T2 transcription termination sequence" misc_feature complement(427..470) /label=rrnB T1 transcription termination sequence /note="rrnB T1 transcription termination sequence" primer_bind 517..533 /label=M13 Forward (-40) primer binding site /note="M13 Forward (-40) primer binding site" primer_bind 537..552 /label=M13 Forward (-20) primer binding site /note="M13 Forward (-20) primer binding site" misc_recomb 570..667 /label=attL3 /note="attL3 recombination site" /note="recognized as attL3" misc_feature 665..691 /label=GGGS linker /note="GGGS linker" gene 692..1432 /label=APEX2 /note="APEX2" /note="derived from Addgene #49386" terminator 1479..2076 /note="WPRE" misc_recomb complement(2101..2198) /label=attL2 /note="attL2 recombination site" /note="recognized as attL2" misc_feature join(2110..3988,1..658) /label=nonstandard type: vector /note="/source=pDONR221-P3P2" /note="/type=Donor Vector" primer_bind complement(2253..2269) /label=M13 Reverse primer binding site /note="M13 Reverse primer binding site" gene 2382..3191 /label=Kanamycin resistance gene /note="Kanamycin resistance gene" rep_origin 3312..3985 /label=f1 origin /note="f1 origin" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataataa 661 agttggtggc ggatccggcg gaggtggatc taagtcttac ccaactgtga gtgctgatta 721 ccaggacgcc gttgagaagg cgaagaagaa gctcagaggc ttcatcgctg agaagagatg 781 cgctcctcta atgctccgtt tggcattcca ctctgctgga acctttgaca agggcacgaa 841 gaccggtgga cccttcggaa ccatcaagca ccctgccgaa ctggctcaca gcgctaacaa 901 cggtcttgac atcgctgtta ggcttttgga gccactcaag gcggagttcc ctattttgag 961 ctacgccgat ttctaccagt tggctggcgt tgttgccgtt gaggtcacgg gtggacctaa 1021 ggttccattc caccctggaa gagaggacaa gcctgagccg ccaccagagg gtcgcttgcc 1081 cgatcccact aagggttctg accatttgag agatgtgttt ggcaaagcta tggggcttac 1141 tgaccaagat atcgttgctc tatctggggg tcacactatt ggagctgcac acaaggagcg 1201 ttctggattt gagggtccct ggacctctaa tcctcttatt ttcgacaact catacttcac 1261 ggagttgttg agtggtgaga aggaaggtct ccttcagcta ccttctgaca aggctctttt 1321 gtctgaccct gtattccgcc ctctcgttga caaatatgca gcggacgaag atgccttctt 1381 tgctgattac gctgaggctc accaaaagct ttccgagctt gggtttgctg atgcctaaga 1441 attcctagag ctcctatagt gagaccggtt agtaatgatc gacaatcaac ctctggatta 1501 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg 1561 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc 1621 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca 1681 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac 1741 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact 1801 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc 1861 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg 1921 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc 1981 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac 2041 gagtcggatc tccctttggg ccgcctcccc gcctggcgat ggtactgtcc tagtacctga 2101 cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa 2161 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg cagggcggcc 2221 gcgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc 2281 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa 2341 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg 2401 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg 2461 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg 2521 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga 2581 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc 2641 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg 2701 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc 2761 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc 2821 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg 2881 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct 2941 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg 3001 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc 3061 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc 3121 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg 3181 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt 3241 gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact 3301 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 3361 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 3421 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 3481 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 3541 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 3601 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 3661 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 3721 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 3781 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 3841 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 3901 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 3961 ccttttgctg gccttttgct cacatgtt //