LOCUS Exported 6099 bp ds-DNA circular SYN 17-JUL-2006 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pDONR221-P3P2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6099) AUTHORS Invitrogen TITLE Direct Submission JOURNAL Exported Jan 17, 2019 from SnapGene Viewer 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6099 /organism="synthetic DNA construct" /note="/dnas_title=JN501_PolyA_EF1a_TETa_GFP_PolyA" misc_feature complement(268..295) /label=rrnB T2 transcription termination sequence /note="rrnB T2 transcription termination sequence" misc_feature complement(427..470) /label=rrnB T1 transcription termination sequence /note="rrnB T1 transcription termination sequence" primer_bind 517..533 /label=M13 Forward (-40) primer binding site /note="M13 Forward (-40) primer binding site" primer_bind 537..552 /label=M13 Forward (-20) primer binding site /note="M13 Forward (-20) primer binding site" misc_recomb 570..665 /label=attL3 /note="attL3 recombination site" /note="recognized as attL3" polyA_signal 708..907 /label=SV40 polyA /note="SV40 polyA" promoter 914..2097 /note="EF1a promoter" promoter 2114..2133 /note="T7 promoter" gene 2177..2877 /label=eGFP /note="eGFP" misc_feature 2894..2902 /label=GSG linker /note="GSG linker" misc_feature 2903..2959 /label=P2A self-cleaving peptide /note="P2A self-cleaving peptide" misc_feature 2954..2959 /label=G^P cleavage site /note="G^P cleavage site" gene 2969..3715 /label=Tet-On Advanced /note="Tet-On Advanced" polyA_signal 3738..4180 /label=SV40 polyA /note="SV40 polyA" misc_recomb complement(4211..4309) /label=attL2 /note="attL2 recombination site" /note="recognized as attL2" misc_feature join(4221..6099,1..658) /label=nonstandard type: vector /note="/source=pDONR221-P3P2" /note="/type=Donor Vector" primer_bind complement(4364..4380) /label=M13 Reverse primer binding site /note="M13 Reverse primer binding site" gene 4493..5302 /label=Kanamycin resistance gene /note="Kanamycin resistance gene" rep_origin 5423..6096 /label=f1 origin /note="f1 origin" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtataataa 661 agttgagatc aacaaagctt cctagctcga tttaaataga taactgacta gaggatcata 721 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc 781 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat 841 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 901 cctcgagtct agacgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 961 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc 1021 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 1081 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 1141 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc 1201 ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt 1261 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt 1321 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt 1381 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct 1441 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt 1501 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg 1561 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc 1621 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg 1681 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca 1741 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa 1801 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg 1861 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg 1921 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca 1981 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc 2041 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga 2101 attagcttgg tactaatacg actcactata gggagaccca agctggctag gtgaattcga 2161 ctggcgcgcc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 2221 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga 2281 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 2341 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga 2401 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 2461 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 2521 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 2581 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa 2641 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt 2701 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 2761 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga 2821 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 2881 gtacaagcgc gccggatcag gagctactaa tttctctctg ttaaagcagg caggagatgt 2941 ggaggagaat ccaggtcctc aattcaccat gtccagactg gacaagagca aagtcataaa 3001 cggcgctctg gaattactca atggagtcgg tatcgaaggc ctgacgacaa ggaaactcgc 3061 tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct 3121 gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct gccccctgga 3181 aggcgagtca tggcaagact ttctgcggaa caacgccaag tcattccgct gtgctctcct 3241 ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga 3301 aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact 3361 gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca 3421 tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag 3481 acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga 3541 actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cggccgacgc 3601 ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga 3661 tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaactaag 3721 taagaatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca 3781 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat 3841 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg 3901 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta 3961 tgatcctgca agcctcgtcg tcctggccgg accacgctat ctgtgcaagg tccccggccc 4021 cggacgcgcg ctccatgagc agagcgcccg ccgccgaggc gaagactcgg gcggcgccct 4081 gcccgtccca ccaggtcaac aggcggtaac cggcctcttc atcgggaatg cgcgcgacct 4141 tcagcatcgc cggcatgtcc ccctggcgga cgggaagtat ccagctcgac caggcttgtc 4201 gactcgaaga acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca 4261 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct 4321 gcagggcggc cgcgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg 4381 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc 4441 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca 4501 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg 4561 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg 4621 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt 4681 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa 4741 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac 4801 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc 4861 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg 4921 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga 4981 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact 5041 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat 5101 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg 5161 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa 5221 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt 5281 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat 5341 tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc 5401 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 5461 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 5521 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 5581 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 5641 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 5701 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 5761 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 5821 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 5881 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 5941 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 6001 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 6061 acggttcctg gccttttgct ggccttttgc tcacatgtt //