LOCUS pDONR221-P4rP3r 4717 bp DNA circular SYN 23-APR-2009 SOURCE ORGANISM COMMENT VNTDATE|391339185| COMMENT VNTDBDATE|416244930| COMMENT VNTNAME|pDONR221-P4rP3r| COMMENT VNTAUTHORNAME|Invitrogen| COMMENT VNTAUTHORTEL|(800)-357-3114| COMMENT VNTAUTHORFAX|(760)-602-6500| COMMENT VNTOAUTHORFTP|ftp://ftp.bioinformatics.invitrogen.com| COMMENT VNTAUTHOREML|bioinfosales@invitrogen.com| COMMENT VNTAUTHORWWW|http://www.invitrogen.com/bioinformatics| COMMENT VNTAUTHORAD1|Invitrogen Corporation| COMMENT VNTAUTHORAD2|1620 Faraday Avenue| COMMENT VNTAUTHORAD3|Carlsbad, CA 92008 U.S.A.| FEATURES Location/Qualifiers misc_recomb complement(570..726) /label=attL4r /note="attL4r recombination site" /note="recognized as attR4" misc_recomb 5586..5746 /label=attL3r /note="attL3r recombination site" /note="recognized as attR3" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence" misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence" primer_bind 537..552 /note="M13 Forward (-20) primer binding site" primer_bind 517..533 /note="M13 Forward (-40) primer binding site" primer_bind complement(5792..5808) /note="M13 Reverse primer binding site" rep_origin 6851..7524 /note="f1 origin" gene 5921..6730 /note="Kanamycin resistance gene" gene 783..3938 /note="hSaCas9 (D10A, N580A) " /note="nuclease deficient" misc_feature 732..782 /note="NLS" misc_feature 3993..5585 /note="VPR" misc_feature 3939..3986 /note="NLS" vector join(5597..7527,1..719) /source="pDONR221-P4rP3r" /type="Donor Vector" misc_feature 4191..4211 /note="SV40 NLS" misc_feature 3993..4178 /note="VP64" misc_feature 4215..4997 /note="p65" misc_feature 4998..5015 /note="GSGSGS linker" misc_feature 5016..5585 /note="Rta" source 1..7527 /dnas_title="Untitled Entry Clone Addgene_68495_AAV_dSaCas9_VPR L4-L3" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccct acaggtcact aataccatct aagtagttga 601 ttcatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa 661 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact tttctatacA 721 AAGTTGccac catggcccca aagaagaagc ggaaggtcgg tatccacgga gtcccagcag 781 ccaagcggaa ctacatcctg ggcctggcca tcggcatcac cagcgtgggc tacggcatca 841 tcgactacga gacacgggac gtgatcgatg ccggcgtgcg gctgttcaaa gaggccaacg 901 tggaaaacaa cgagggcagg cggagcaaga gaggcgccag aaggctgaag cggcggaggc 961 ggcatagaat ccagagagtg aagaagctgc tgttcgacta caacctgctg accgaccaca 1021 gcgagctgag cggcatcaac ccctacgagg ccagagtgaa gggcctgagc cagaagctga 1081 gcgaggaaga gttctctgcc gccctgctgc acctggccaa gagaagaggc gtgcacaacg 1141 tgaacgaggt ggaagaggac accggcaacg agctgtccac caaagagcag atcagccgga 1201 acagcaaggc cctggaagag aaatacgtgg ccgaactgca gctggaacgg ctgaagaaag 1261 acggcgaagt gcggggcagc atcaacagat tcaagaccag cgactacgtg aaagaagcca 1321 aacagctgct gaaggtgcag aaggcctacc accagctgga ccagagcttc atcgacacct 1381 acatcgacct gctggaaacc cggcggacct actatgaggg acctggcgag ggcagcccct 1441 tcggctggaa ggacatcaaa gaatggtacg agatgctgat gggccactgc acctacttcc 1501 ccgaggaact gcggagcgtg aagtacgcct acaacgccga cctgtacaac gccctgaacg 1561 acctgaacaa tctcgtgatc accagggacg agaacgagaa gctggaatat tacgagaagt 1621 tccagatcat cgagaacgtg ttcaagcaga agaagaagcc caccctgaag cagatcgcca 1681 aagaaatcct cgtgaacgaa gaggatatta agggctacag agtgaccagc accggcaagc 1741 ccgagttcac caacctgaag gtgtaccacg acatcaagga cattaccgcc cggaaagaga 1801 ttattgagaa cgccgagctg ctggatcaga ttgccaagat cctgaccatc taccagagca 1861 gcgaggacat ccaggaagaa ctgaccaatc tgaactccga gctgacccag gaagagatcg 1921 agcagatctc taatctgaag ggctataccg gcacccacaa cctgagcctg aaggccatca 1981 acctgatcct ggacgagctg tggcacacca acgacaacca gatcgctatc ttcaaccggc 2041 tgaagctggt gcccaagaag gtggacctgt cccagcagaa agagatcccc accaccctgg 2101 tggacgactt catcctgagc cccgtcgtga agagaagctt catccagagc atcaaagtga 2161 tcaacgccat catcaagaag tacggcctgc ccaacgacat cattatcgag ctggcccgcg 2221 agaagaactc caaggacgcc cagaaaatga tcaacgagat gcagaagcgg aaccggcaga 2281 ccaacgagcg gatcgaggaa atcatccgga ccaccggcaa agagaacgcc aagtacctga 2341 tcgagaagat caagctgcac gacatgcagg aaggcaagtg cctgtacagc ctggaagcca 2401 tccctctgga agatctgctg aacaacccct tcaactatga ggtggaccac atcatcccca 2461 gaagcgtgtc cttcgacaac agcttcaaca acaaggtgct cgtgaagcag gaagaagcca 2521 gcaagaaggg caaccggacc ccattccagt acctgagcag cagcgacagc aagatcagct 2581 acgaaacctt caagaagcac atcctgaatc tggccaaggg caagggcaga atcagcaaga 2641 ccaagaaaga gtatctgctg gaagaacggg acatcaacag gttctccgtg cagaaagact 2701 tcatcaaccg gaacctggtg gataccagat acgccaccag aggcctgatg aacctgctgc 2761 ggagctactt cagagtgaac aacctggacg tgaaagtgaa gtccatcaat ggcggcttca 2821 ccagctttct gcggcggaag tggaagttta agaaagagcg gaacaagggg tacaagcacc 2881 acgccgagga cgccctgatc attgccaacg ccgatttcat cttcaaagag tggaagaaac 2941 tggacaaggc caaaaaagtg atggaaaacc agatgttcga ggaaaagcag gccgagagca 3001 tgcccgagat cgaaaccgag caggagtaca aagagatctt catcaccccc caccagatca 3061 agcacattaa ggacttcaag gactacaagt acagccaccg ggtggacaag aagcctaata 3121 gagagctgat taacgacacc ctgtactcca cccggaagga cgacaagggc aacaccctga 3181 tcgtgaacaa tctgaacggc ctgtacgaca aggacaatga caagctgaaa aagctgatca 3241 acaagagccc cgaaaagctg ctgatgtacc accacgaccc ccagacctac cagaaactga 3301 agctgattat ggaacagtac ggcgacgaga agaatcccct gtacaagtac tacgaggaaa 3361 ccgggaacta cctgaccaag tactccaaaa aggacaacgg ccccgtgatc aagaagatta 3421 agtattacgg caacaaactg aacgcccatc tggacatcac cgacgactac cccaacagca 3481 gaaacaaggt cgtgaagctg tccctgaagc cctacagatt cgacgtgtac ctggacaatg 3541 gcgtgtacaa gttcgtgacc gtgaagaatc tggatgtgat caaaaaagaa aactactacg 3601 aagtgaatag caagtgctat gaggaagcta agaagctgaa gaagatcagc aaccaggccg 3661 agtttatcgc ctccttctac aacaacgatc tgatcaagat caacggcgag ctgtatagag 3721 tgatcggcgt gaacaacgac ctgctgaacc ggatcgaagt gaacatgatc gacatcacct 3781 accgcgagta cctggaaaac atgaacgaca agaggccccc caggatcatt aagacaatcg 3841 cctccaagac ccagagcatt aagaagtaca gcacagacat tctgggcaac ctgtatgaag 3901 tgaaatctaa gaagcaccct cagatcatca aaaagggcaa aaggccggcg gccacgaaaa 3961 aggccggcca ggcaaaaaag aaaaagggat ccgaggccag cggttccgga cgggctgacg 4021 cattggacga ttttgatctg gatatgctgg gaagtgacgc cctcgatgat tttgaccttg 4081 acatgcttgg ttcggatgcc cttgatgact ttgacctcga catgctcggc agtgacgccc 4141 ttgatgattt cgacctggac atgctgatta actctagaag ttccggatct ccgaaaaaga 4201 aacgcaaagt tggtagccag tacctgcccg acaccgacga ccggcaccgg atcgaggaaa 4261 agcggaagcg gacctacgag acattcaaga gcatcatgaa gaagtccccc ttcagcggcc 4321 ccaccgaccc tagacctcca cctagaagaa tcgccgtgcc cagcagatcc agcgccagcg 4381 tgccaaaacc tgccccccag ccttacccct tcaccagcag cctgagcacc atcaactacg 4441 acgagttccc taccatggtg ttccccagcg gccagatctc tcaggcctct gctctggctc 4501 cagcccctcc tcaggtgctg cctcaggctc ctgctcctgc accagctcca gccatggtgt 4561 ctgcactggc tcaggcacca gcacccgtgc ctgtgctggc tcctggacct ccacaggctg 4621 tggctccacc agcccctaaa cctacacagg ccggcgaggg cacactgtct gaagctctgc 4681 tgcagctgca gttcgacgac gaggatctgg gagccctgct gggaaacagc accgatcctg 4741 ccgtgttcac cgacctggcc agcgtggaca acagcgagtt ccagcagctg ctgaaccagg 4801 gcatccctgt ggcccctcac accaccgagc ccatgctgat ggaatacccc gaggccatca 4861 cccggctcgt gacaggcgct cagaggcctc ctgatccagc tcctgcccct ctgggagcac 4921 caggcctgcc taatggactg ctgtctggcg acgaggactt cagctctatc gccgatatgg 4981 atttctcagc cttgctgggc tctggcagcg gcagccggga ttccagggaa gggatgtttt 5041 tgccgaagcc tgaggccggc tccgctatta gtgacgtgtt tgagggccgc gaggtgtgcc 5101 agccaaaacg aatccggcca tttcatcctc caggaagtcc atgggccaac cgcccactcc 5161 ccgccagcct cgcaccaaca ccaaccggtc cagtacatga gccagtcggg tcactgaccc 5221 cggcaccagt ccctcagcca ctggatccag cgcccgcagt gactcccgag gccagtcacc 5281 tgttggagga tcccgatgaa gagacgagcc aggctgtcaa agcccttcgg gagatggccg 5341 atactgtgat tccccagaag gaagaggctg caatctgtgg ccaaatggac ctttcccatc 5401 cgcccccaag gggccatctg gatgagctga caaccacact tgagtccatg accgaggatc 5461 tgaacctgga ctcacccctg accccggaat tgaacgagat tctggatacc ttcctgaacg 5521 acgagtgcct cttgcatgcc atgcatatca gcacaggact gtccatcttc gacacatctc 5581 tgtttGGAGC AACTTTgtat aataaagttg aacgagaaac gtaaaatgat ataaatatca 5641 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata 5701 tccagtcact atgaatcaac tacttagatg gtattagtga cctgtactgc agggcggccg 5761 cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc 5821 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat 5881 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga 5941 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc 6001 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc 6061 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat 6121 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg 6181 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt 6241 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg 6301 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct 6361 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga 6421 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc 6481 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg 6541 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct 6601 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca 6661 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga 6721 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg 6781 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg 6841 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt 6901 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 6961 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 7021 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 7081 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 7141 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 7201 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 7261 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 7321 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 7381 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 7441 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 7501 cttttgctgg ccttttgctc acatgtt //