LOCUS Exported 8131 bp ds-DNA circular SYN 19-DEC-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8131) AUTHORS . TITLE Direct Submission JOURNAL Exported Jan 17, 2019 from SnapGene Viewer 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8131 /organism="synthetic DNA construct" /note="/dnas_title=IY601_pLenti_Blast" LTR 1..410 /label=RSV/5' LTR hybrid promoter /note="RSV/5' LTR hybrid promoter" misc_feature 520 /label=5' splice donor /note="5' splice donor" misc_feature 521..565 /label=HIV-1 psi packaging signal /note="HIV-1 psi packaging signal" misc_feature 1075..1308 /label=HIV-1 Rev response element /note="HIV-1 Rev response element" misc_feature 1656 /label=3' splice acceptor /note="3' splice acceptor" misc_feature 1684 /label=3' splice acceptor /note="3' splice acceptor" misc_feature 1801..1923 /label=cPPT /note="cPPT" misc_recomb 1978..2105 /label=attR1 /note="attR1" misc_feature complement(2140..2162) /label=nonstandard type: PCR_primer /note="JH43" /note="Invitrogen Primer 1" /note="sequencing primer" gene 2214..2873 /label=Chloramphenicol resistance gene /note="Chloramphenicol resistance gene" gene 3215..3520 /label=ccdB gene /note="ccdB gene" misc_feature 3421..3443 /label=nonstandard type: PCR_primer /note="JH44" /note="Invitrogen Primer 2" /note="sequencing primer" misc_recomb 3561..3685 /label=attR2 /note="attR2" promoter 3737..4045 /note="SV40 promoter" promoter 4100..4166 /note="EM7 promoter" gene 4167..4565 /label=Blasticidin resistance gene /note="Blasticidin resistance gene" LTR 4651..4885 /label=3' LTR /note="3' LTR" polyA_signal 4957..5088 /label=SV40 polyA signal /note="SV40 polyA signal" promoter 5947..6045 /note="bla promoter" gene 6046..6906 /label=Ampicillin resistance gene /note="Ampicillin resistance gene" rep_origin 7051..7724 /label=pUC origin /note="pUC origin" ORIGIN 1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca 61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga 121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt 181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg 241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg 421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt 481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga 961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct 1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg 1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgataag cttgtctaga ggatccacta gtccagtgtg gtggaattct gcagatatca 1981 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta 2041 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca 2101 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg 2161 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga 2221 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg 2281 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg 2341 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc 2401 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg 2461 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt 2521 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag 2581 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt 2641 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata 2701 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg 2761 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca 2821 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg 2881 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta 2941 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca 3001 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc 3061 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa 3121 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac 3181 ggctcttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa 3241 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg 3301 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga 3361 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc 3421 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga 3481 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca 3541 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag 3601 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc 3661 gttcagcttt cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag 3721 aggtggcgat ggttccggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 3781 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 3841 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 3901 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 3961 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga 4021 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt 4081 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat 4141 aatacgacaa ggtgaggaac taaaccatgg ccaagccttt gtctcaagaa gaatccaccc 4201 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg 4261 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta 4321 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca 4381 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac 4441 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg 4501 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg 4561 gctaagcaca attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat 4621 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga 4681 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag 4741 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt 4801 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt 4861 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta 4921 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat 4981 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 5041 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc 5101 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 5161 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 5221 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag 5281 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 5341 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 5401 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga 5461 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 5521 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 5581 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 5641 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 5701 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 5761 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 5821 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 5881 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg 5941 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 6001 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 6061 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 6121 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 6181 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 6241 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 6301 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 6361 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 6421 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 6481 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 6541 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 6601 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 6661 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 6721 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 6781 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 6841 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 6901 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 6961 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 7021 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 7081 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 7141 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 7201 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag 7261 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 7321 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 7381 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 7441 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 7501 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 7561 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 7621 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 7681 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 7741 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 7801 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc 7861 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 7921 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 7981 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 8041 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac 8101 taaagggaac aaaagctgga gctgcaagct t //