LOCUS       Exported                5900 bp ds-DNA     circular SYN 19-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 17, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=KJ800_pMVP_Hygro"
     promoter        30..128
                     /note="bla promoter"
     gene            129..989
                     /label=Ampicillin
                     /note="Ampicillin"
     rep_origin      1134..1807
                     /label=pUC origin
                     /note="pUC origin"
     misc_feature    1925..1942
                     /label=nonstandard type: PCR_primer
                     /note="M13F(-21)"
     misc_feature    1989..2007
                     /label=nonstandard type: PCR_primer
                     /note="JH637"
                     /note="sequencing primer"
     misc_recomb     2008..2132
                     /label=attR1
                     /note="attR1"
     misc_feature    complement(2167..2189)
                     /label=nonstandard type: PCR_primer
                     /note="JH43"
                     /note="Invitrogen Primer 1"
                     /note="sequencing primer"
     gene            2241..2900
                     /label=Chlor
                     /note="Chlor"
                     /note="Chloramphenicol resistance gene"
     gene            3242..3547
                     /label=ccdB
                     /note="ccdB"
                     /note="ccdB gene"
     misc_feature    3448..3470
                     /label=nonstandard type: PCR_primer
                     /note="JH44"
                     /note="Invitrogen Primer 2"
                     /note="sequencing primer"
     misc_recomb     3588..3712
                     /label=attR2
                     /note="attR2"
     misc_feature    complement(3745..3763)
                     /label=nonstandard type: PCR_primer
                     /note="JH638"
                     /note="sequencing primer"
     promoter        3764..4072
                     /note="SV40 early promoter and origin"
     misc_feature    3993..4012
                     /label=nonstandard type: PCR_primer
                     /note="JH619"
                     /note="sequencing primer"
     promoter        4127..4193
                     /note="EM7 promoter"
     gene            4194..5231
                     /gene="Hygromycin resistance gene"
                     /label=Hygromycin resistance gene
     polyA_signal    5258..5529
                     /label=TK Poly A signal
                     /note="TK Poly A signal"
     misc_feature    complement(5661..5678)
                     /label=nonstandard type: PCR_primer
                     /note="JH618"
                     /note="sequencing primer"
     misc_feature    complement(5714..5731)
                     /label=nonstandard type: PCR_primer
                     /note="JH622"
                     /note="sequencing primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagctgtaaa acgacggcca gtagctccac cgcggtggcg gccgctctag aactagtgga
     1981 tcccccgggc tgcaggaatt cgatatcaca agtttgtaca aaaaagctga acgagaaacg
     2041 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
     2101 tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta
     2161 cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt
     2221 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
     2281 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
     2341 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
     2401 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc
     2461 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
     2521 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
     2581 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
     2641 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
     2701 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
     2761 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
     2821 ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
     2881 agtggcaggg cggggcgtaa acgcgtggat ccggcttact aaaagccaga taacagtatg
     2941 cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta
     3001 tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta
     3061 tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag
     3121 aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct
     3181 gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga
     3241 aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt
     3301 acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg
     3361 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag
     3421 ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt
     3481 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
     3541 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga
     3601 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
     3661 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatatcaa
     3721 gcttatcgat accgtcgacc tcgacatggt catagctgtt gacggtgtgg aaagtcccca
     3781 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     3841 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3901 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     3961 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     4021 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     4081 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
     4141 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatgaaga
     4201 agcccgaact caccgctacc agcgttgaaa aatttctcat cgagaagttc gacagtgtga
     4261 gcgacctgat gcagttgtcg gagggcgaag agagccgagc cttcagcttc gatgtcggcg
     4321 gacgcggcta tgtactgcgg gtgaatagct gcgctgatgg cttctacaaa gaccgctacg
     4381 tgtaccgcca cttcgccagc gctgcactac ccatccccga agtgttggac atcggcgagt
     4441 tcagcgagag cctgacatac tgcatcagta gacgcgccca aggcgttact ctccaagacc
     4501 tccccgaaac agagctgcct gctgtgttac agcctgtcgc cgaagctatg gatgctattg
     4561 ccgccgccga cctcagtcaa accagcggct tcggcccatt cgggccccaa ggcatcggcc
     4621 agtacacaac ctggcgggat ttcatttgcg ccattgctga tccccatgtc taccactggc
     4681 agaccgtgat ggacgacacc gtgtccgcca gcgtagctca agccctggac gaactgatgc
     4741 tgtgggccga agactgtccc gaggtgcgcc acctcgtcca tgccgacttc ggcagcaaca
     4801 acgtcctgac cgacaacggc cgcatcaccg ccgtaatcga ctggtccgaa gctatgttcg
     4861 gggacagtca gtacgaggtg gccaacatct tcttctggcg gccctggctg gcttgcatgg
     4921 agcagcagac tcgctacttc gagcgccggc atcccgagct ggccggcagc cctcgtctgc
     4981 gagcctacat gctgcgcatc ggcctggatc agctctacca gagcctcgtg gacggcaact
     5041 tcgacgatgc tgcctgggct caaggccgct gcgatgccat cgtccgcagc ggggccggca
     5101 ccgtcggtcg cacacaaatc gctcgccgga gcgcagccgt atggaccgac ggctgcgtcg
     5161 aggtgctggc cgacagcggc aaccgccggc ccagtacacg accgcgcgct aaggaggtag
     5221 gtcgagttta acgtaccggt tagtaatgag tttaaacggg ggaggctaac tgaaacacgg
     5281 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
     5341 cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     5401 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     5461 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
     5521 tgccatagca gatccggtta aatcaaaaga atagaccgag atagggttga gtgttgttcc
     5581 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     5641 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     5701 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     5761 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     5821 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     5881 gccgctacag ggcgcgtcag
//
