LOCUS Exported 5237 bp ds-DNA circular SYN 19-DEC-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5237) AUTHORS . TITLE Direct Submission JOURNAL Exported Jan 17, 2019 from SnapGene Viewer 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5237 /organism="synthetic DNA construct" /note="/dnas_title=KK401_pMVP_Zeo" promoter 30..128 /note="bla promoter" gene 129..989 /label=Ampicillin /note="Ampicillin" rep_origin 1134..1807 /label=pUC origin /note="pUC origin" misc_feature 1925..1942 /label=nonstandard type: PCR_primer /note="M13F(-21)" misc_feature 1989..2007 /label=nonstandard type: PCR_primer /note="JH637" /note="sequencing primer" misc_recomb 2008..2132 /label=attR1 /note="attR1" misc_feature complement(2167..2189) /label=nonstandard type: PCR_primer /note="JH43" /note="Invitrogen Primer 1" /note="sequencing primer" gene 2241..2900 /label=Chlor /note="Chlor" /note="Chloramphenicol resistance gene" gene 3242..3547 /label=ccdB /note="ccdB" /note="ccdB gene" misc_feature 3448..3470 /label=nonstandard type: PCR_primer /note="JH44" /note="Invitrogen Primer 2" /note="sequencing primer" misc_recomb 3588..3712 /label=attR2 /note="attR2" misc_feature complement(3745..3763) /label=nonstandard type: PCR_primer /note="JH638" /note="sequencing primer" promoter 3764..4072 /note="SV40 early promoter and origin" misc_feature 3993..4012 /label=nonstandard type: PCR_primer /note="JH619" /note="sequencing primer" promoter 4127..4193 /note="EM7 promoter" gene 4194..4568 /label=Zeocin resistance gene /note="Zeocin resistance gene" polyA_signal 4595..4866 /label=TK Poly A signal /note="TK Poly A signal" misc_feature complement(4998..5015) /label=nonstandard type: PCR_primer /note="JH618" /note="sequencing primer" misc_feature complement(5051..5068) /label=nonstandard type: PCR_primer /note="JH622" /note="sequencing primer" ORIGIN 1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 1921 aagctgtaaa acgacggcca gtagctccac cgcggtggcg gccgctctag aactagtgga 1981 tcccccgggc tgcaggaatt cgatatcaca agtttgtaca aaaaagctga acgagaaacg 2041 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa 2101 tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta 2161 cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt 2221 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat 2281 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata 2341 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca 2401 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc 2461 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg 2521 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc 2581 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt 2641 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca 2701 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg 2761 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg 2821 ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg 2881 agtggcaggg cggggcgtaa acgcgtggat ccggcttact aaaagccaga taacagtatg 2941 cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta 3001 tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta 3061 tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag 3121 aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct 3181 gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga 3241 aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt 3301 acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg 3361 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag 3421 ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt 3481 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg 3541 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga 3601 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat 3661 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatatcaa 3721 gcttatcgat accgtcgacc tcgacatggt catagctgtt gacggtgtgg aaagtcccca 3781 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 3841 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 3901 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 3961 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct 4021 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 4081 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat 4141 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca 4201 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct 4261 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc 4321 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc 4381 tggcctgggt gttggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt 4441 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg 4501 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc 4561 aggactgacg taccggttag taatgagttt aaacggggga ggctaactga aacacggaag 4621 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac 4681 gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga 4741 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca 4801 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc 4861 catagcagat ccggttaaat caaaagaata gaccgagata gggttgagtg ttgttccagt 4921 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 4981 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 5041 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 5101 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 5161 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 5221 gctacagggc gcgtcag //