LOCUS       Exported                4587 bp ds-DNA     circular SYN 19-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 17, 2019 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=JN601_pMVP_SB_DEST"
     promoter        30..128
                     /note="bla promoter"
     gene            129..989
                     /label=Ampicillin
                     /note="Ampicillin"
     rep_origin      1134..1807
                     /label=pUC origin
                     /note="pUC origin"
     misc_feature    1925..1942
                     /label=nonstandard type: PCR_primer
                     /note="M13F(-21) *DON'T USE*"
                     /note="Doesn't prime efficiently"
     misc_feature    1986..1989
                     /label=tata
                     /note="tata"
     misc_feature    1990..2214
                     /label=Sleeping Beauty 5' ITR
                     /note="Sleeping Beauty 5' ITR"
                     /note="derived from from Addgene 26557"
     misc_feature    2222..2240
                     /label=nonstandard type: PCR_primer
                     /note="JH637"
                     /note="sequencing primer"
     misc_recomb     2241..2365
                     /label=attR1
                     /note="attR1"
     misc_feature    complement(2400..2422)
                     /label=nonstandard type: PCR_primer
                     /note="JH43"
                     /note="Invitrogen Primer 1"
                     /note="sequencing primer"
     gene            2474..3133
                     /label=Chlor
                     /note="Chlor"
                     /note="Chloramphenicol resistance gene"
     gene            3475..3780
                     /label=ccdB
                     /note="ccdB"
                     /note="ccdB gene"
     misc_feature    3681..3703
                     /label=nonstandard type: PCR_primer
                     /note="JH44"
                     /note="Invitrogen Primer 2"
                     /note="sequencing primer"
     misc_recomb     3821..3945
                     /label=attR2
                     /note="attR2"
     misc_feature    complement(3978..3996)
                     /label=nonstandard type: PCR_primer
                     /note="JH638"
                     /note="sequencing primer"
     misc_feature    4072..4299
                     /label=Sleeping Beauty 3' ITR
                     /note="Sleeping Beauty 3' ITR"
                     /note="Derived from Addgene 26557"
     misc_feature    4300..4303
                     /label=tata
                     /note="tata"
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    complement(4348..4365)
                     /label=nonstandard type: PCR_primer
                     /note="JH618"
                     /note="sequencing primer"
     misc_feature    complement(4401..4418)
                     /label=nonstandard type: PCR_primer
                     /note="JH622"
                     /note="sequencing primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagctgtaaa acgacggcca gtagctccac cgcggtggcg gccgctctag aactagtgta
     1981 caccctatac agttgaagtc ggaagtttac atacacttaa gttggagtca ttaaaactcg
     2041 tttttcaact actccacaaa tttcttgtta acaaacaata gttttggcaa gtcagttagg
     2101 acatctactt tgtgcatgac acaagtcatt tttccaacaa ttgtttacag acagattatt
     2161 tcacttataa ttcactgtat cacaattcca gtgggtcaga agtttacata cactaagttg
     2221 ggctgcagga attcgatatc acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg
     2281 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
     2341 aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta
     2401 tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc
     2461 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
     2521 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
     2581 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     2641 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc
     2701 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     2761 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
     2821 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     2881 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
     2941 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
     3001 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
     3061 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
     3121 gggcggggcg taaacgcgtg gatccggctt actaaaagcc agataacagt atgcgtattt
     3181 gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa
     3241 aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg
     3301 ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag
     3361 cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg
     3421 cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag
     3481 tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt
     3541 gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg
     3601 tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc
     3661 atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat
     3721 ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa
     3781 atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg
     3841 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
     3901 tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgatat caagcttatc
     3961 gataccgtcg acctcgacat ggtcatagct gttgacctcg agttaaatca aaagaataga
     4021 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactgtac attgagtgta
     4081 tgtaaacttc tgacccactg ggaatgtgat gaaagaaata aaagctgaaa tgaatcattc
     4141 tctctactat tattctgata tttcacattc ttaaaataaa gtggtgatcc taactgacct
     4201 aagacaggga atttttacta ggattaaatg tcaggaattg tgaaaaagtg agtttaaatg
     4261 tatttggcta aggtgtatgt aaacttccga cttcaactgt atagggattg tacaaagggc
     4321 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     4381 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     4441 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     4501 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     4561 ttaatgcgcc gctacagggc gcgtcag
//
