LOCUS Exported 6037 bp ds-DNA circular SYN 19-DEC-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6037) AUTHORS . TITLE Direct Submission JOURNAL Exported Jan 17, 2019 from SnapGene Viewer 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6037 /organism="synthetic DNA construct" /note="/dnas_title=KN300_pMVP_SB_CFP_DEST" promoter 30..128 /note="bla promoter" gene 129..989 /label=Ampicillin /note="Ampicillin" rep_origin 1134..1807 /label=pUC origin /note="pUC origin" misc_feature 1925..1942 /label=nonstandard type: PCR_primer /note="M13F(-21) *DON'T USE*" /note="Doesn't prime efficiently" misc_feature 1986..1989 /label=tata /note="tata" misc_feature 1990..2214 /label=Sleeping Beauty 5' ITR /note="Sleeping Beauty 5' ITR" /note="derived from from Addgene 26557" misc_feature 2222..2240 /label=nonstandard type: PCR_primer /note="JH637" /note="sequencing primer" misc_recomb 2241..2365 /label=attR1 /note="attR1" misc_feature complement(2400..2422) /label=nonstandard type: PCR_primer /note="JH43" /note="Invitrogen Primer 1" /note="sequencing primer" gene 2474..3133 /label=Chlor /note="Chlor" /note="Chloramphenicol resistance gene" gene 3475..3780 /label=ccdB /note="ccdB" /note="ccdB gene" misc_feature 3681..3703 /label=nonstandard type: PCR_primer /note="JH44" /note="Invitrogen Primer 2" /note="sequencing primer" misc_recomb 3821..3945 /label=attR2 /note="attR2" misc_feature complement(3978..3996) /label=nonstandard type: PCR_primer /note="JH638" /note="sequencing primer" promoter 3997..4305 /note="SV40 early promoter and origin" misc_feature 4226..4245 /label=nonstandard type: PCR_primer /note="JH619" /note="sequencing primer" promoter 4360..4426 /note="EM7 promoter" gene 4427..5146 /label=eCFP /note="eCFP" polyA_signal 5173..5444 /label=TK Poly A signal /note="TK Poly A signal" misc_feature 5522..5749 /label=Sleeping Beauty 3' ITR /note="Sleeping Beauty 3' ITR" /note="Derived from Addgene 26557" misc_feature 5750..5753 /label=tata /note="tata" misc_feature complement(5798..5815) /label=nonstandard type: PCR_primer /note="JH618" /note="sequencing primer" misc_feature complement(5851..5868) /label=nonstandard type: PCR_primer /note="JH622" /note="sequencing primer" ORIGIN 1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 1921 aagctgtaaa acgacggcca gtagctccac cgcggtggcg gccgctctag aactagtgta 1981 caccctatac agttgaagtc ggaagtttac atacacttaa gttggagtca ttaaaactcg 2041 tttttcaact actccacaaa tttcttgtta acaaacaata gttttggcaa gtcagttagg 2101 acatctactt tgtgcatgac acaagtcatt tttccaacaa ttgtttacag acagattatt 2161 tcacttataa ttcactgtat cacaattcca gtgggtcaga agtttacata cactaagttg 2221 ggctgcagga attcgatatc acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg 2281 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta 2341 aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta 2401 tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc 2461 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg 2521 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac 2581 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta 2641 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc 2701 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca 2761 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt 2821 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa 2881 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt 2941 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata 3001 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg 3061 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca 3121 gggcggggcg taaacgcgtg gatccggctt actaaaagcc agataacagt atgcgtattt 3181 gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa 3241 aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg 3301 ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag 3361 cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg 3421 cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag 3481 tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt 3541 gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg 3601 tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc 3661 atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat 3721 ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa 3781 atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg 3841 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt 3901 tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgatat caagcttatc 3961 gataccgtcg acctcgacat ggtcatagct gttgacggtg tggaaagtcc ccaggctccc 4021 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 4081 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 4141 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 4201 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg 4261 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 4321 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc 4381 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg tgagcaaggg 4441 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg 4501 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 4561 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct 4621 gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt 4681 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 4741 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 4801 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 4861 ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa 4921 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 4981 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca 5041 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 5101 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaacgta ccggttagta 5161 atgagtttaa acgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc 5221 gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat 5281 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg 5341 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc 5401 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcagatcc ggttaaatca 5461 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactgtac 5521 attgagtgta tgtaaacttc tgacccactg ggaatgtgat gaaagaaata aaagctgaaa 5581 tgaatcattc tctctactat tattctgata tttcacattc ttaaaataaa gtggtgatcc 5641 taactgacct aagacaggga atttttacta ggattaaatg tcaggaattg tgaaaaagtg 5701 agtttaaatg tatttggcta aggtgtatgt aaacttccga cttcaactgt atagggattg 5761 tacaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa 5821 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc 5881 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg 5941 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca 6001 cccgccgcgc ttaatgcgcc gctacagggc gcgtcag //