LOCUS Exported 5692 bp ds-DNA circular SYN 19-DEC-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5692) AUTHORS . TITLE Direct Submission JOURNAL Exported Jan 17, 2019 from SnapGene Viewer 4.2.11 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5692 /organism="synthetic DNA construct" /note="/dnas_title=KK901_pMVP_SB_Zeo" promoter 30..128 /note="bla promoter" gene 129..989 /label=Ampicillin /note="Ampicillin" rep_origin 1134..1807 /label=pUC origin /note="pUC origin" misc_feature 1925..1942 /label=nonstandard type: PCR_primer /note="M13F(-21) *DON'T USE*" /note="Doesn't prime efficiently" misc_feature 1986..1989 /label=tata /note="tata" misc_feature 1990..2214 /label=Sleeping Beauty 5' ITR /note="Sleeping Beauty 5' ITR" /note="derived from from Addgene 26557" misc_feature 2222..2240 /label=nonstandard type: PCR_primer /note="JH637" /note="sequencing primer" misc_recomb 2241..2365 /label=attR1 /note="attR1" misc_feature complement(2400..2422) /label=nonstandard type: PCR_primer /note="JH43" /note="Invitrogen Primer 1" /note="sequencing primer" gene 2474..3133 /label=Chlor /note="Chlor" /note="Chloramphenicol resistance gene" gene 3475..3780 /label=ccdB /note="ccdB" /note="ccdB gene" misc_feature 3681..3703 /label=nonstandard type: PCR_primer /note="JH44" /note="Invitrogen Primer 2" /note="sequencing primer" misc_recomb 3821..3945 /label=attR2 /note="attR2" misc_feature complement(3978..3996) /label=nonstandard type: PCR_primer /note="JH638" /note="sequencing primer" promoter 3997..4305 /note="SV40 early promoter and origin" misc_feature 4226..4245 /label=nonstandard type: PCR_primer /note="JH619" /note="sequencing primer" promoter 4360..4426 /note="EM7 promoter" gene 4427..4801 /label=Zeocin resistance gene /note="Zeocin resistance gene" polyA_signal 4828..5099 /label=TK Poly A signal /note="TK Poly A signal" misc_feature 5177..5404 /label=Sleeping Beauty 3' ITR /note="Sleeping Beauty 3' ITR" /note="Derived from Addgene 26557" misc_feature 5405..5408 /label=tata /note="tata" misc_feature complement(5453..5470) /label=nonstandard type: PCR_primer /note="JH618" /note="sequencing primer" misc_feature complement(5506..5523) /label=nonstandard type: PCR_primer /note="JH622" /note="sequencing primer" ORIGIN 1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 1921 aagctgtaaa acgacggcca gtagctccac cgcggtggcg gccgctctag aactagtgta 1981 caccctatac agttgaagtc ggaagtttac atacacttaa gttggagtca ttaaaactcg 2041 tttttcaact actccacaaa tttcttgtta acaaacaata gttttggcaa gtcagttagg 2101 acatctactt tgtgcatgac acaagtcatt tttccaacaa ttgtttacag acagattatt 2161 tcacttataa ttcactgtat cacaattcca gtgggtcaga agtttacata cactaagttg 2221 ggctgcagga attcgatatc acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg 2281 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta 2341 aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta 2401 tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc 2461 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg 2521 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac 2581 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta 2641 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc 2701 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca 2761 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt 2821 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa 2881 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt 2941 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata 3001 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg 3061 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca 3121 gggcggggcg taaacgcgtg gatccggctt actaaaagcc agataacagt atgcgtattt 3181 gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa 3241 aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg 3301 ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag 3361 cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg 3421 cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag 3481 tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt 3541 gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg 3601 tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc 3661 atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat 3721 ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa 3781 atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg 3841 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt 3901 tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgatat caagcttatc 3961 gataccgtcg acctcgacat ggtcatagct gttgacggtg tggaaagtcc ccaggctccc 4021 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 4081 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 4141 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 4201 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg 4261 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 4321 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc 4381 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac 4441 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga 4501 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga 4561 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg 4621 ggtgttggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa 4681 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga 4741 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg 4801 acgtaccggt tagtaatgag tttaaacggg ggaggctaac tgaaacacgg aaggagacaa 4861 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt 4921 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 4981 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 5041 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagca 5101 gatccggtta aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac 5161 aagagtccac tgtacattga gtgtatgtaa acttctgacc cactgggaat gtgatgaaag 5221 aaataaaagc tgaaatgaat cattctctct actattattc tgatatttca cattcttaaa 5281 ataaagtggt gatcctaact gacctaagac agggaatttt tactaggatt aaatgtcagg 5341 aattgtgaaa aagtgagttt aaatgtattt ggctaaggtg tatgtaaact tccgacttca 5401 actgtatagg gattgtacaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 5461 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 5521 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 5581 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 5641 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ag //