LOCUS       LB901_pMVP_PB_Bl        5823 bp    DNA     circular     19-DEC-2018
FEATURES             Location/Qualifiers
     misc_recomb     2263..2387
                     /note="attR1"
     PCR_primer      complement(2422..2444)
                     /note="JH43"
                     /note="Invitrogen Primer 1"
                     /note="sequencing primer"
     gene            2496..3155
                     /note="Chlor"
                     /note="Chloramphenicol resistance gene"
     PCR_primer      3703..3725
                     /note="JH44"
                     /note="Invitrogen Primer 2"
                     /note="sequencing primer"
     gene            3497..3802
                     /note="ccdB"
                     /note="ccdB gene"
     misc_recomb     3843..3967
                     /note="attR2"
     gene            129..989
                     /note="Ampicillin"
     promoter        30..128
                     /note="bla promoter"
     rep_origin      1134..1807
                     /note="pUC origin"
     PCR_primer      1944..1961
                     /note="M13F(-21) *DON'T USE*"
                     /note="Doesn't prime efficiently"
     PCR_primer      complement(5584..5601)
                     /note="JH618"
                     /note="sequencing primer"
     PCR_primer      complement(5637..5654)
                     /note="JH622"
                     /note="sequencing primer"
     misc_feature    5226..5538
                     /note="PiggyBac 5' ITR and insulator"
                     /note="derived from Addgene #63800"
     repeat_region   5500..5534
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal
                     repeat sequence (ITR)"
     misc_feature    5535..5538
                     /note="ttaa"
     repeat_region   1987..2049
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal
                     repeat sequence (ITR)"
     misc_feature    1983..2217
                     /note="PiggyBac 3' ITR and insulator"
                     /note="derived from Addgene #63800"
     misc_feature    1983..1986
                     /note="TTAA"
     promoter        4019..4327
                     /note="SV40 early promoter and origin"
     promoter        4382..4448
                     /note="EM7 promoter"
     gene            4449..4847
                     /note="Blasticidin resistance gene"
     polyA_signal    4874..5145
                     /note="TK Poly A signal"
     PCR_primer      2244..2262
                     /note="JH637"
                     /note="sequencing primer"
     PCR_primer      complement(4000..4018)
                     /note="JH638"
                     /note="sequencing primer"
     PCR_primer      4248..4267
                     /note="JH619"
                     /note="sequencing primer"
     source          1..5823
                     /dnas_title="LB901_pMVP_PB_Blast_DEST"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCACATGct gtacagctta agtcatgTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT
     1861 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC
     1921 AGCGAGTCAG TGAGCGAGGA agcTGTAAAA CGACGGCCAG TagctCCACC GCcgcagcta
     1981 gattaaccct agaaagataa tcatattgtg acgtacgtta aagataatca tgcgtaaaat
     2041 tgacgcatgt gttttatcgg tctgtatatc gaggtttatt tattaatttg aatagatatt
     2101 aagttttatt atatttacac ttacatacta ataataaatt caacaaacaa tttatttatg
     2161 tttatttatt tattaaaaaa aaacaaaaac tcaaaatttc ttctataaag taacaaaact
     2221 tttatgACTA GTGGATCCCC CGGGCTGCAG GAATTCGATA TCACAAGTTT GTACAAAAAA
     2281 GCTGAACGAG AAACGTAAAA TGATATAAAT ATCAATATAT TAAATTAGAT TTTGCATAAA
     2341 AAACAGACTA CATAATACTG TAAAACACAA CATATCCAGT CACTATGGCG GCCGCATTAG
     2401 GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATAA TGTGTGGATT TTGAGTTAGG
     2461 ATCCGTCGAG ATTTTCAGGA GCTAAGGAAG CTAAAATGGA GAAAAAAATC ACTGGATATA
     2521 CCACCGTTGA TATATCCCAA TGGCATCGTA AAGAACATTT TGAGGCATTT CAGTCAGTTG
     2581 CTCAATGTAC CTATAACCAG ACCGTTCAGC TGGATATTAC GGCCTTTTTA AAGACCGTAA
     2641 AGAAAAATAA GCACAAGTTT TATCCGGCCT TTATTCACAT TCTTGCCCGC CTGATGAATG
     2701 CTCATCCGGA ATTCCGTATG GCAATGAAAG ACGGTGAGCT GGTGATATGG GATAGTGTTC
     2761 ACCCTTGTTA CACCGTTTTC CATGAGCAAA CTGAAACGTT TTCATCGCTC TGGAGTGAAT
     2821 ACCACGACGA TTTCCGGCAG TTTCTACACA TATATTCGCA AGATGTGGCG TGTTACGGTG
     2881 AAAACCTGGC CTATTTCCCT AAAGGGTTTA TTGAGAATAT GTTTTTCGTC TCAGCCAATC
     2941 CCTGGGTGAG TTTCACCAGT TTTGATTTAA ACGTGGCCAA TATGGACAAC TTCTTCGCCC
     3001 CCGTTTTCAC CATGGGCAAA TATTATACGC AAGGCGACAA GGTGCTGATG CCGCTGGCGA
     3061 TTCAGGTTCA TCATGCCGTT TGTGATGGCT TCCATGTCGG CAGAATGCTT AATGAATTAC
     3121 AACAGTACTG CGATGAGTGG CAGGGCGGGG CGTAAACGCG TGGATCCGGC TTACTAAAAG
     3181 CCAGATAACA GTATGCGTAT TTGCGCGCTG ATTTTTGCGG TATAAGAATA TATACTGATA
     3241 TGTATACCCG AAGTATGTCA AAAAGAGGTA TGCTATGAAG CAGCGTATTA CAGTGACAGT
     3301 TGACAGCGAC AGCTATCAGT TGCTCAAGGC ATATATGATG TCAATATCTC CGGTCTGGTA
     3361 AGCACAACCA TGCAGAATGA AGCCCGTCGT CTGCGTGCCG AACGCTGGAA AGCGGAAAAT
     3421 CAGGAAGGGA TGGCTGAGGT CGCCCGGTTT ATTGAAATGA ACGGCTCTTT TGCTGACGAG
     3481 AACAGGGGCT GGTGAAATGC AGTTTAAGGT TTACACCTAT AAAAGAGAGA GCCGTTATCG
     3541 TCTGTTTGTG GATGTACAGA GTGATATTAT TGACACGCCC GGGCGACGGA TGGTGATCCC
     3601 CCTGGCCAGT GCACGTCTGC TGTCAGATAA AGTCTCCCGT GAACTTTACC CGGTGGTGCA
     3661 TATCGGGGAT GAAAGCTGGC GCATGATGAC CACCGATATG GCCAGTGTGC CGGTCTCCGT
     3721 TATCGGGGAA GAAGTGGCTG ATCTCAGCCA CCGCGAAAAT GACATCAAAA ACGCCATTAA
     3781 CCTGATGTTC TGGGGAATAT AAATGTCAGG CTCCCTTATA CACAGCCAGT CTGCAGGTCG
     3841 ACCATAGTGA CTGGATATGT TGTGTTTTAC AGTATTATGT AGTCTGTTTT TTATGCAAAA
     3901 TCTAATTTAA TATATTGATA TTTATATCAT TTTACGTTTC TCGTTCAGCT TTCTTGTACA
     3961 AAGTGGTGAT ATCAAGCTTA TCGATACCGT CGACCtcgaC ATGGTCATAG CTGTTgacGG
     4021 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG
     4081 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG
     4141 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT
     4201 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG
     4261 GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC
     4321 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAGCAC
     4381 GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC AAGGTGAGGA
     4441 ACTAAACCAT GGCCAAGCCT TTGTCTCAAG AAGAATCCAC CCTCATTGAA AGAGCAACGG
     4501 CTACAATCAA CAGCATCCCC ATCTCTGAAG ACTACAGCGT CGCCAGCGCA GCTCTCTCTA
     4561 GCGACGGCCG CATCTTCACT GGTGTCAATG TATATCATTT TACTGGGGGA CCTTGTGCAG
     4621 AACTCGTGGT GCTGGGCACT GCTGCTGCTG CGGCAGCTGG CAACCTGACT TGTATCGTCG
     4681 CGATCGGAAA TGAGAACAGG GGCATCTTGA GCCCCTGCGG ACGGTGCCGA CAGGTGCTTC
     4741 TCGATCTGCA TCCTGGGATC AAAGCCATAG TGAAGGACAG TGATGGACAG CCGACGGCAG
     4801 TTGGGATTCG TGAATTGCTG CCCTCTGGTT ATGTGTGGGA GGGCTAACGT ACCGGTTAGT
     4861 AATGAGTTTA AACGGGGGAG GCTAACTGAA ACACGGAAGG AGACAATACC GGAAGGAACC
     4921 CGCGCTATGA CGGCAATAAA AAGACAGAAT AAAACGCACG GGTGTTGGGT CGTTTGTTCA
     4981 TAAACGCGGG GTTCGGTCCC AGGGCTGGCA CTCTGTCGAT ACCCCACCGA GACCCCATTG
     5041 GGGCCAATAC GCCCGCGTTT CTTCCTTTTC CCCACCCCAC CCCCCAAGTT CGGGTGAAGG
     5101 CCCAGGGCTC GCAGCCAACG TCGGGGCGGC AGGCCCTGCC ATAGCAGATC CGgtTAAATC
     5161 AAAAGAATAG ACCGAGATAG GGTTGAGTGT TGTTCCAGTT TGGAACAAGA GTCCACgctg
     5221 tccctgatat ctataacaag aaaatatata tataataagt tatcacgtaa gtagaacatg
     5281 aaataacaat ataattatcg tatgagttaa atcttaaaag tcacgtaaaa gataatcatg
     5341 cgtcattttg actcacgcgg tcgttatagt tcaaaatcag tgacacttac cgcattgaca
     5401 agcacgcctc acgggagctc caagcggcga ctgagatgtc ctaaatgcac agcgacggat
     5461 tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga
     5521 ctatctttct agggttaatc tagctgcatc AAGGGCGAAA AACCGTCTAT CAGGGCGATG
     5581 GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC
     5641 TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG
     5701 TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG
     5761 CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT
     5821 CAG
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