LOCUS       BirA_insert_eCPX        5918 bp ds-DNA     linear       21-DEC-2018
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    346..1329
                     /locus_tag="BirA"
                     /label="BirA"
                     /ApEinfo_label="BirA"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     source          complement(join(3524..3724,4352..4459))
                     /organism="Flexi vector pF1K T7"
                     /mol_type="other DNA"
                     /db_xref="taxon:303938"
                     /locus_tag="source:Flexi vector pF1K T7"
                     /label="source:Flexi vector pF1K T7"
                     /ApEinfo_label="source:Flexi vector pF1K T7"
                     /ApEinfo_fwdcolor="#ffffff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1391..1413
                     /locus_tag="pTrcHis rev primer"
                     /label="pTrcHis rev primer"
                     /ApEinfo_label="pTrcHis rev primer"
                     /ApEinfo_fwdcolor="#d65f30"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(4420..4439)
                     /note="T7 RNA polymerase promoter"
                     /locus_tag="T7 RNA polymerase promoter"
                     /label="T7 RNA polymerase promoter"
                     /ApEinfo_label="T7 RNA polymerase promoter"
                     /ApEinfo_fwdcolor="#3189ba"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4460..4478
                     /locus_tag="Reverse primer"
                     /label="Reverse primer"
                     /ApEinfo_label="Reverse primer"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(join(3725..3748,3752..4351))
                     /locus_tag="eCPX"
                     /label="eCPX"
                     /ApEinfo_label="eCPX"
                     /ApEinfo_fwdcolor="#ba1a28"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(join(3734..3748,3752..3778))
                     /locus_tag="AviTag"
                     /label="AviTag"
                     /ApEinfo_label="AviTag"
                     /ApEinfo_fwdcolor="#249312"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3457..3493
                     /locus_tag="ColEI"
                     /label="ColEI"
                     /ApEinfo_label="ColEI"
                     /ApEinfo_fwdcolor="#d65f30"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      complement(3549..3596)
                     /note="T7 terminator"
                     /locus_tag="T7 terminator"
                     /label="T7 terminator"
                     /ApEinfo_label="T7 terminator"
                     /ApEinfo_fwdcolor="#70ff48"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3505..3523
                     /locus_tag="Forward primer"
                     /label="Forward primer"
                     /ApEinfo_label="Forward primer"
                     /ApEinfo_fwdcolor="#249312"
                     /ApEinfo_revcolor="#00ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3749..3751
                     /locus_tag="K to A mutation"
                     /label="K to A mutation"
                     /ApEinfo_label="K to A mutation"
                     /ApEinfo_fwdcolor="#66ffff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT
       61 TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA
      121 AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG
      181 ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG
      241 ATCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG
      301 CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACA TACCCATGAA GGATAACACC
      361 GTGCCACTGA AATTGATTGC CCTGTTAGCG AACGGTGAAT TTCACTCTGG CGAGCAGTTG
      421 GGTGAAACGC TGGGAATGAG CCGGGCGGCT ATTAATAAAC ACATTCAGAC ACTGCGTGAC
      481 TGGGGCGTTG ATGTCTTTAC CGTTCCGGGT AAAGGATACA GCCTGCCTGA GCCTATCCAG
      541 TTACTTAATG CTAAACAGAT ATTGGGTCAG CTGGATGGCG GTAGTGTAGC CGTGCTGCCA
      601 GTGATTGACT CCACGAATCA GTACCTTCTT GATCGTATCG GAGAGCTTAA ATCGGGCGAT
      661 GCTTGCATTG CAGAATACCA GCAGGCTGGC CGTGGTCGCC GGGGTCGGAA ATGGTTTTCG
      721 CCTTTTGGCG CAAACTTATA TTTGTCGATG TTCTGGCGTC TGGAACAAGG CCCGGCGGCG
      781 GCGATTGGTT TAAGTCTGGT TATCGGTATC GTGATGGCGG AAGTATTACG CAAGCTGGGT
      841 GCAGATAAAG TTCGTGTTAA ATGGCCTAAT GACCTCTATC TGCAGGATCG CAAGCTGGCA
      901 GGCATTCTGG TGGAGCTGAC TGGCAAAACT GGCGATGCGG CGCAAATAGT CATTGGAGCC
      961 GGGATCAACA TGGCAATGCG CCGTGTTGAA GAGAGTGTCG TTAATCAGGG GTGGATCACG
     1021 CTGCAGGAAG CGGGGATCAA TCTCGATCGT AATACGTTGG CGGCCATGCT AATACGTGAA
     1081 TTACGTGCTG CGTTGGAACT CTTCGAACAA GAAGGATTGG CACCTTATCT GTCGCGCTGG
     1141 GAAAAGCTGG ATAATTTTAT TAATCGCCCA GTGAAACTTA TCATTGGTGA TAAAGAAATA
     1201 TTTGGCATTT CACGCGGAAT AGACAAACAG GGGGCTTTAT TACTTGAGCA GGATGGAATA
     1261 ATAAAACCCT GGATGGGCGG TGAAATATCC CTGCGTAGTG CAGAAAAACA TCATCATCAT
     1321 CATCATTGAG AGTTTAAACG GTCTCCAGCT TGGCTGTTTT GGCGGATGAG AGAAGATTTT
     1381 CAGCCTGATA CAGATTAAAT CAGAACGCAG AAGCGGTCTG ATAAAACAGA ATTTGCCTGG
     1441 CGGCAGTAGC GCGGTGGTCC CACCTGACCC CATGCCGAAC TCAGAAGTGA AACGCCGTAG
     1501 CGCCGATGGT AGTGTGGGGT CTCCCCATGC GAGAGTAGGG AACTGCCAGG CATCAAATAA
     1561 AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG
     1621 CTCTCCTGAG TAGGACAAAT CCGCCGGGAG CGGATTTGAA CGTTGCGAAG CAACGGCCCG
     1681 GAGGGTGGCG GGCAGGACGC CCGCCATAAA CTGCCAGGCA TCAAATTAAG CAGAAGGCCA
     1741 TCCTGACGGA TGGCCTTTTT GCGTTTCTAC AAACTCTTTT TGTTTATTTT TCTAAATACA
     1801 TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA
     1861 AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT
     1921 TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA
     1981 GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG
     2041 TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC
     2101 GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA
     2161 GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT
     2221 AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT
     2281 GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT
     2341 AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA
     2401 CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT
     2461 TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC
     2521 ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA
     2581 GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT
     2641 AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA
     2701 GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT
     2761 TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA
     2821 TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT
     2881 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA
     2941 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT
     3001 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA
     3061 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT
     3121 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC
     3181 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA
     3241 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA
     3301 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG
     3361 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT
     3421 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG
     3481 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGaaccgga tatagttcct
     3541 cctttcagca aaaaacccct caagacccgt ttagaggccc caaggggtta tgctagttat
     3601 tgctcagcgg tggcagcagc caactcagct tcctttcggg ctttgttagc agccggatca
     3661 gcttgcatgc ctgcaggtcg actctagagg atccccgggt accgagctcg aattcgttta
     3721 aacaTTACTT AAGCTCGTGC CACTCGATTG CCTGTGCTTC AAAAATATCA TTCAGACCGC
     3781 GGCCGCCTGC ACGACGTGCA CCGCTTGCGG TACGGCTTTT TTCGGTATAG GTAAAGCTAC
     3841 CAATAACACC CAGCGGACTA TTATCTTCTT CGTAGCGATA TTTCAGATTA AAGCCACCCA
     3901 TTTTATTCAT CTGACCCTGT GCATCACTCT GTGCATAACC ACCGGTAACG GTGCTGGTTG
     3961 CACGACGGCT TTTGCTACCA AAACGATAAC CAACGCTCAG AATCCAGGTG CCAACATCAA
     4021 CGCTACGAAT ACGGCTCTGT TCATAGCTGA AATCCAGTGC AACATTTTCC ATCGGATTAA
     4081 ACTGCAGACC TGCACCATAG CTAAAACCAT AATCGCTGGT ATCATGTTTA TAGGTCGGAT
     4141 ATTCGGTGGT CTGAAATTTA CCATAACCAA CACCCACAAC ACCATAAATG CTTGCCCAAT
     4201 CATTAATACG ATATGCCGGA CCGGCTGTAA TGCCGTAATA CTGATTTTTG TTATAATCAC
     4261 CACTCTGACC GCTCTGACCA CCGGCAACGC TGGTGCCTGC GGTAAATGCC AGAACTGCTG
     4321 CCAGTGCGCT CAGACATGCG ATTTTTTTCA Tggcgatcgc tccttactaa agttaaagaa
     4381 aattattact agtggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa
     4441 tttcgcggga tcgagatccC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT
     4501 CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA
     4561 GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT
     4621 ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATGGTGCACT CTCAGTACAA
     4681 TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT
     4741 CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT
     4801 CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT
     4861 TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCAGATCAAT TCGCGCGCGA AGGCGAAGCG
     4921 GCATGCATAA TGTGCCTGTC AAATGGACGA AGCAGGGATT CTGCAAACCC TATGCTACTC
     4981 CGTCAAGCCG TCAATTGTCT GATTCGTTAC CAATTATGAC AACTTGACGG CTACATCATT
     5041 CACTTTTTCT TCACAACCGG CACGGAACTC GCTCGGGCTG GCCCCGGTGC ATTTTTTAAA
     5101 TACCCGCGAG AAATAGAGTT GATCGTCAAA ACCAACATTG CGACCGACGG TGGCGATAGG
     5161 CATCCGGGTG GTGCTCAAAA GCAGCTTCGC CTGGCTGATA CGTTGGTCCT CGCGCCAGCT
     5221 TAAGACGCTA ATCCCTAACT GCTGGCGGAA AAGATGTGAC AGACGCGACG GCGACAAGCA
     5281 AACATGCTGT GCGACGCTGG CGATATCAAA ATTGCTGTCT GCCAGGTGAT CGCTGATGTA
     5341 CTGACAAGCC TCGCGTACCC GATTATCCAT CGGTGGATGG AGCGACTCGT TAATCGCTTC
     5401 CATGCGCCGC AGTAACAATT GCTCAAGCAG ATTTATCGCC AGCAGCTCCG AATAGCGCCC
     5461 TTCCCCTTGC CCGGCGTTAA TGATTTGCCC AAACAGGTCG CTGAAATGCG GCTGGTGCGC
     5521 TTCATCCGGG CGAAAGAACC CCGTATTGGC AAATATTGAC GGCCAGTTAA GCCATTCATG
     5581 CCAGTAGGCG CGCGGACGAA AGTAAACCCA CTGGTGATAC CATTCGCGAG CCTCCGGATG
     5641 ACGACCGTAG TGATGAATCT CTCCTGGCGG GAACAGCAAA ATATCACCCG GTCGGCAAAC
     5701 AAATTCTCGT CCCTGATTTT TCACCACCCC CTGACCGCGA ATGGTGAGAT TGAGAATATA
     5761 ACCTTTCATT CCCAGCGGTC GGTCGATAAA AAAATCGAGA TAACCGTTGG CCTCAATCGG
     5821 CGTTAAACCC GCCACCAGAT GGGCATTAAA CGAGTATCCC GGCAGCAGGG GATCATTTTG
     5881 CGCTTCAGCC ATACTTTTCA TACTCCCGCC ATTCAGAG  
//
